摘要
目的 :研究p2 7kip1基因对人食管癌细胞EC -970 6的DNA复制及蛋白质合成的影响。方法 :重组体腺病毒Ad -p2 7kip1转染EC -970 6细胞 ,采用细胞生长计数、[3H] -TdR、[3H] -Leucine掺入法、流式细胞术 ,研究其对食管癌细胞DNA复制及蛋白合成的影响。结果 :Ad -p2 7kip1组细胞生长明显受抑 ,[3H] -TdR和 [3H ] -Leucine掺入量均明显减少 ,与对照组比较P <0 0 1;食管癌细胞的增殖明显受抑制。结论 :p2 7可有效抑制食管癌细胞的增殖活性 ,这与其抑制食管癌细胞DNA复制及蛋白质合成有关。
AIM: To investigate the effects of p27kip1 ge ne on DNA replication and protein synthesis in esophageal carcinoma cells. METHODS: Recombinant adenovirus Ad-p27kip1 was constructed and transf ected into esophageal carcinoma cell EC-9706, and its effects on DNA replication , protein synthesis and the growth of esophageal carcinoma cells were explored b y means of cell growth count, -TdR, -Leucine incorporation meth od and flow cytometry. RESULTS: The growth of esophageal carcino ma cells was inhibited obviously. The radioactive intensity of -TdR and -Leucine in Ad-p27kip1 group decreased significantly than that in contr ol group after EC-9706 transfection (P<0 01), while the multiplication of e sophageal carcinoma cell was inhibited obviously. CONCLUSION: Ad -p27kip1 inhibited the multiplication of the esophageal carcinoma cells, which m ay be related to its suppressive function for DNA replication and protein synthesis .
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2004年第1期100-102,共3页
Chinese Journal of Pathophysiology
