摘要
目的 :研究拟胆碱药卡巴胆碱对缺血再灌注肠道局部炎症反应的影响及意义。方法 :成年雄性Wistar大鼠 ,戊巴比妥钠腹腔麻醉 ,先制作 8cm长的空肠袋 ,后用动脉夹阻断肠系膜上动脉 (SMAO)血流 ,1 h后松夹恢复灌流 ,制成肠道缺血再灌注模型 ,将模型动物随机分为预防组、治疗组和对照组 ,预防组和治疗组分别在夹闭后 30 m in和复流后 30 m in向空肠袋内注射卡巴胆碱 (0 .1 m g/ kg,稀释到 1 m l生理盐水中 ) ,对照组仅注射生理盐水。各组分别于夹闭后 1 .0、2 .5和 6 .0 h分别处死动物 ,测定肠袋组织肿瘤坏死因子 α(TNFα)和髓过氧化物酶 (MPO)含量 ,并观察肠道组织病理形态学改变。结果 :预防组和治疗组肠组织中TNFα和 MPO含量显著低于对照组 (P均 <0 .0 5 ) ;肠道组织炎性损害明显减轻 ;治疗组与预防组之间上述变化差异不显著。结论 :卡巴胆碱能减轻缺血再灌注大鼠肠道局部炎症反应 ,机制可能与其对抗致炎因子的作用有关。
Objective: To investigate the effects of carbachol on local inflammation in gut tissue during ischemia/reperfusion(I/R) . Methods: A jejunal sac was formed in Wistar rats. The superior mesenteric artery was occluded (SMAO) for 60 minutes followed by reperfusion for another 60 minutes. Animals were divided into three groups, pretreatment group (carbachol was injected into the jejunal sac 30 minutes after SMAO, 0 1 mg/kg ), treatment group (carbachol was injected in same dosage into the jejunal sac 30 minutes after reperfusion ), and controls (saline injection). The contents of tumor necrosis factorα(TNFα) and activity of myeloperoxidase(MPO) in gut tissue were determined at 1 hours, 2 5 hours and 6 hours after SMAO. Results : The contents of TNFα and activity of MPO were significantly decreased in pretreatment and treatment groups compared with control group at 2 5 hours after SMAO (both P <0 05 ). There were no differences in both contents between pretreatment group and treatment group at any specified time. It was also found that there were less inflammatory pathological changes in the gut tissues in the two treated groups than that of control. Conclusion: The results suggest that carbachol could alleviate gut inflammatory response during gut ischemia/reperfusion injury by inhibiting proinflammatory cytokine release.
出处
《中国危重病急救医学》
CAS
CSCD
2003年第12期748-750,共3页
Chinese Critical Care Medicine
基金
全军医学科学技术研究"十五"计划指令性课题基金资助项目 ( 0 1L 0 81)
