摘要
采用单因素与正交试验结合的方法对白芷叶总香豆素的大孔树脂纯化工艺进行研究,发现其优选工艺为:大孔树脂型号为D-101、上柱液的质量浓度为0.2 g/mL、上柱体积为3 BV,上柱液pH为4,上柱液流速为2 BV/h,洗脱用乙醇体积分数为90%,洗脱速度为2 BV/h,洗脱体积为6 BV.采用该纯化工艺,可使白芷叶总香豆素的纯度达到58.3%.
The method of combining single factor with orthogonal test was used to study the purification process of macroporous resin from total coumarin of Angelica Dahurica leaves.The optimized process is as follows:the macroporous resin model is D-101,the concentration of the sample solution is 0.2 g/mL,the volume of the supernatant is 3 BV,the pH of the sample is 4,the loading speed is 2 BV/h,the ethanol volume fraction is 90%,the elution rate is 2 BV/h and the elution volume is 6 BV.Using the optimized process,total coumarin purity of Angelica Dahurica leaves is up to 58.3%.
作者
薛天乐
王庆
栗进才
刘耀武
XUE Tianle;WANG Qing;LI Jincai;LIU Yaowu(College of Pharmacy,Bozhou Vocational and Technicel College,Bozhou,Anhui 236800,China;Bozhou Institute of Chinese Medicine,Anhui Academy of Chinese Medicine,Bozhou,Anhui 236800,China)
出处
《宜宾学院学报》
2019年第6期116-120,共5页
Journal of Yibin University
基金
安徽教育厅自然科学基金重大项目(KJ2018ZD065)
安徽省教育厅自然科学重点课题(KJ2018A0885)
关键词
白芷叶
总香豆素
大孔树脂
纯化工艺
单因素与正交试验
Angelica Dahurica leaves
total coumarin
macroporous resin
purification process
single factor and orthogonal test
