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邻菲咯啉浓度对瘤胃微生物体外发酵的影响 被引量:3

THE INFLUENCE OF1,10-PHENANTHROLINE CONCENTRATIONS ON IN VITRO FERMENTATION OF MIXED RUMINAL MICROORGANISMS
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摘要 邻菲咯啉是一种二肽酶抑制剂。本试验用体外法测定了不同浓度邻菲咯啉对瘤胃微生物发酵产气量、氨氮浓度、真蛋白含量及饲料干物质降解率的影响。结果表明 ,邻菲咯啉浓度与产气量呈直线负相关(Y=-16.75X +29.52 ,r=0.7976,P<0.05);与发酵液氨氮浓度呈直线负相关(Y= -0.5757X +3.3036,r=0.8384,P<0.05) ;与发酵管内容物真蛋白含量呈直线正相关(Y=1.35X +11.45,r=0.8423,P<0.05) ;与内容物饲料干物质降解率呈直线负相关(Y= -10.14X +55.08,r=0.9275,P<0.01)。 Gas production,NH 3 -N concentration in the incubation fluid,true protein content,and DM degradability were measured when different amount of1,10-phenanthroline were supplemented in a in vitro fermentation experiment using mixed ruminal microorganisms.A mixture of0.3g of graund peanut vines and mixed concentrate was added to each tube.The result indicated that phenanthroline inhibited gas production and NH 3 -N concentration severely.The negative liner regression equation between phenanthroline concentration and gas production obtained was Y=-16.75X+29.52(r=0.7976,P<0.05),and the equation between phenanthroline concentration and NH 3 -N concentration was Y=-0.5757X+3.3036(r=0.8384,P<0.05).True protein content increased with phenanthroline concentraˉtion,and the regression equation was Y=1.35X+11.45(r=0.8423,P<0.05).DM degradadility deˉcreased with phenanthroline concentration(Y=-10.14X+55.08,r=0.9275,P<0.01)。
出处 《动物营养学报》 CAS CSCD 2003年第4期28-31,43,共5页 CHINESE JOURNAL OF ANIMAL NUTRITION
关键词 邻菲咯啉 浓度 瘤胃微生物 体外发酵 二肽酶抑制剂 产气量 真蛋白 氨氮浓度 饲料干物质 phenanthroline In vitro fermentation gas production true protein NH 3 -N concentration
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  • 1冯宗慈,高民.通过比色测定瘤胃液氨氮含量方法的改进[J].内蒙古畜牧科学,1993(4):40-41. 被引量:424
  • 2Mehrez A Z. 1980. Processing factors affecting degradability of fish meal in the rumen[J].J Anita. Sci., 50:737.
  • 3Schaefer D M, Davis C L, Bryant M P. 1980. Ammonia saturation constants for predominant species of tureen bacteria[J ]. J Dairy Sci. 63(8) : 1248 - 1263.
  • 4Wallace R J, Mckain N. 1996 . Influence of 1, 10- phenanthroline and its analogues other chelators and transition metal ions on dipeptidase activity of the rumen bacterium prevotella ruminicola[ J ]. J Appl. Bacteriol ., 81 (1):42-47.

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