摘要
目的 :研究全身炎症反应综合征 (SIRS)早期和晚期即多器官功能障碍综合征 (MODS)期中性粒细胞(PMN )基因表达的差异 ,并探讨其功能的改变。方法 :应用基因芯片技术对 4例 SIRS患者早期和晚期外周血PMN的 RNA进行检测 ,观察并分析其表达的差异性。结果 :在 84 6 4条基因中发现差异表达基因 84条 ,其中19条上调 ,6 5条下调 ,差异表达的基因主要是细胞受体基因 (31% )、免疫相关基因 (2 7% )、代谢相关基因(2 0 % )以及 DNA结合与转录的各种因子基因 (18% )等。结论 :MODS期 PMN基因的差异表达提示 PMN处于功能障碍状态 ,即参加先天免疫反应的能力减弱而自身组织损伤增强 ,这可能预示患者预后差。
Objective: To study the gene expression difference of polymorphonuclear neutrophils(PMN) in systemic inflammatory response syndrome(SIRS) in early and late periods termed as multiple organ dysfunction syndrome(MODS) and to evaluate the related changes in function of PMN. Methods: Using cDNA microarray technology, RNA of peripheral blood PMN of 4 patients with SIRS was detected in early and late periods, the gene expression difference was obserred and analyzed. Results: Among 8 464 genes there were 84 differently expressed with 19 expressed higher and 65 expressed lower. The differently expressed genes consisted of cell receptor genes(31 percent),immunity-related genes(27 percent), metabolism-related genes(20 percent), and genes of DNA binding or transcriptional factors (18 percent) etc. Conclusion: Differently expressed genes suggest that PMN in MODS be in a dysfunctional state characterized by decreasing innate immunity response and increased tissue auto-injury, which may portend a bad prognosis of the patients.
出处
《中国危重病急救医学》
CAS
CSCD
2003年第11期651-654,共4页
Chinese Critical Care Medicine
基金
上海市曙光计划资助项目 ( 0 1SG3 3 )
关键词
全身炎症反应综合征
多器官功能障碍综合征
中性粒细胞
基因芯片
systemic inflammatory response syndrome
multiple organ dysfunction syndrome
polymorphonuclear neutrophil
cDNA microarray
