摘要
目的 :了解不同乙型肝炎病毒 (HBV)感染患者血清HBV -DNA含量与乙肝血清免疫标志物的关系并探讨其临床意义。方法 :用荧光定量聚合酶链反应 (FQ -PCR)方法定量检测 375例不同临床类型HBV感染者及 70例正常人血清HBV -DNA含量。结果 :血清HBsAg阳性组与HBeAg阳性组HBV -DNA的检出率及含量均明显高于HBsAg阴性组和HBeAg阴性组 ,且HBeAg即使阴性 ,不论HBeAb阳性组或阴性组均仍有较高的HBV -DNA检出率。不同临床类型HBV感染者中均有HBV -DNA的检出。结论 :定量PCR可真实反映HBV感染、复制及病程变化 ,对乙型肝炎临床诊断及治疗均有一定的临床意义。
Objective To study the relationship between the serum content of HBV-DNA and expression of serological markers with HBV infection patients. Methods Serum samples from 375 hepatitis B patients with different clinical status and 70 normal persons were quantitated for HBV-DNA by FQ-PCR. Results The average of HBV-DNA contents in the patient in the groups of HBsAg(+) and of HBeAg(+) were significantly higher than those in the group of HBsAg(-) and of HBeAg(-). Even in the group of HBeAg negative, high HBV-DNA contents might still be present in both the HBeAg(+) and HBeAg(-) groups. Conclusion FQ-PCR can be used to monitor the real state of HBV infection, replication and the course of disease.
出处
《放射免疫学杂志》
CAS
2003年第5期310-312,共3页
Journal of Radioimmanology
