摘要
以壳寡糖为诱导物处理烟草植株,利用高效液相色谱 电化学(库仑电极)阵列检测技术检测不同处理时间植物体内内源激素的变化情况及其他小分子物质的差异显示。所用色谱柱为HypersilBDSC18柱(4.6mmi d ×200mm,5μm),八通道电化学阵列检测器(八个电极)的检测电势依次为-200,300,400,500,600,700,800,850mV。结果表明,茉莉酸(JA)和赤霉酸(GA)含量在用壳寡糖处理6h时达到高峰,吲哚乙酸(IAA)含量在处理8h时达到高峰。处理后烟草植株产生的其他一些未知的小分子物质尚有待定性和进一步考察其变化规律。
Oligochitosan was used as elicitor to treat tobacco leaves, and the concentrations of internal secretions and low molecular weight compounds were determined by high performance liquid chromatography with a coulometric array detector (HPLCCAD). The CAD has 8 electrochemical detector cells and the cell potentials were maintained at -200, 300, 400, 500, 600, 700, 800, 850 mV, beginning with the first sensor in series. A Hypersil BDS C18 chromatographic column (4.6 mm id×200 mm, 5 μm) was employed. The concentrations changed with the treating time by oligochitosan: jasmonic acid (JA) and gibberellic acid (GA) reached peak value after being treated for 6 h, whereas indoleacetic acid (IAA) did for 8 h. Some other compounds was observed as well during the treatment. They are believed to be low molecular weight compounds and subject to further investigations.
出处
《色谱》
CAS
CSCD
北大核心
2003年第5期507-509,共3页
Chinese Journal of Chromatography
基金
国家自然科学基金项目(39980012)
国家高技术研究发展计划(863计划)项目(2001AA620501)
(2002AA245131).
关键词
高效液相色谱
电化学阵列检测
植物
内源激素
小分子物质
差异显示
库仑电极
壳寡糖
oligochitosan
high performance liquid chromatography
coulometric array detection
internal secretion
low molecular weight compound
differential display
