摘要
建立芒果中杀菌剂啶酰菌胺和吡唑醚菌酯残留检测的超高效液相色谱方法。芒果样品经乙腈提取,氨基(NH2)固相萃取柱净化,以乙腈和水为流动相梯度洗脱,采用双波长紫外检测器检测,外标法定量。结果表明:啶酰菌胺和吡唑醚菌酯含量分别在0.10~4.00μg/mL和0.02~1.00μg/mL质量浓度范围内与色谱峰面积呈现良好的线性关系,决定系数R2均大于0.999。方法的检出限均为0.002 mg/kg,分别从3个不同添加水平检测结果可以看出。方法的回收率在76.00%~103.00%之间,相对标准偏差为1.07%~13.28%。
An ultra performance liquid chromatography(UPLC) method was established for the determination of boscalid and pyraclostrobin in mango. Samples were extracted with acetonitrile, purified on NH2 column, and analyzed by gradient elution using acetonitrile-water as the mobile phase. The residues were determined by dual-wavelength ultraviolet detector, and quantified by an external standard method. An excellent linearity for boscalid and pyraclostrobin was observed in the range of 0.10–4.00 μg/mL and 0.02–1.00 μg/mL with correlation coefficients higher than 0.999, respectively. The limit of detection for both pesticides was 0.002 mg/kg. The average recovery rate for the pesticides from mongo at three spiked concentrations was in the range of 76.00%–103.00% with relative standard deviations of 1.07%–13.28%.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2014年第20期175-179,共5页
Food Science
基金
公益性行业(农业)科研专项(201303088-13)
关键词
超高效液相色谱
芒果
啶酰菌胺
吡唑醚菌酯
ultra performance liquid chromatography(UPLC)
mango
boscalid
pyraclostrobin
