摘要
目的 :HPLC法测定骨刺宁酊中胡椒碱的含量。方法 :高效液相色谱法 ,采用ODS柱 ,以甲醇 -水 (70∶30 )为流动相 ,检测波长为 343nm。结果 :胡椒碱在 0 .3μg～ 1.6 μg线性关系良好 (r =0 .9996 ) ,平均加样回收率为98.8% ,RSD =2 .98%。结论 :该方法精密度高 ,分离度、重复性良好 ,结果准确可靠 。
Objective: To determine the content of the pepper alkali in the peaceful tincture of spur with HPLC law. Methods: With high-efficient liquid phase chromatogram , ODS column was adopted with the methyl alcohol - water( 70∶30)as flowing phases, measuring wavelength as 343nm. Results: The pepper alkali had good linear relations of 0.3 μg～1.6 μg line =0.9996), added kinds of rate of recovery was 98.8%, RSD equally =2.98%. Conclusion: The method has high precision, good separating degree, good repetitiveness , accurate and reliable results have received, so it can be used in the quality control of the peaceful tincture of spur. This law used in this preparation has not seen reported.
