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植物基因工程疫苗高效表达载体的构建 被引量：3

On Constructing Expressing Vectors for Genetic Engineering Vaccine
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摘要 分别将编码HIV-1的gag基因、gp120基因及gag-gp120嵌合基因,FMDV的P1全长基因和猪白细胞介素18(IL18)基因克隆到含CaMV35S启动子控制下的双元表达载体pBI121中,并通过冻融法导入农杆菌LBA4404,成功构建了能在植物中高效表达的5种植物表达载体:pBI121gag,pBI121gp,pBI121gg,pBI121P1,pBI121IL18。 The main antigen gene encoded HIV-1, FMDV and IL18 were cloned into pBI121 vector including CaMV35S promoter, then transferred into Agrobacterium tumefaciens LBA4404 using the freezethaw method?Therefore five efficient expression vectors were successfully constructed It made a good foundation for producing new gene engineering vaccines using transgenic plants as bioreactors
作者 李昌 金宁一 王罡 张洪勇 王萍 季静 郑敏
机构地区 解放军军需大学植物基因工程研究中心
出处 《吉林农业大学学报》 CAS CSCD 北大核心 2003年第3期253-256,共4页 Journal of Jilin Agricultural University
基金 863项目"(2001AA213071) "国家植物转基因中试及产业化基地"专项基金资助项目(J99-B-001)
关键词 基因工程疫苗 表达载体 抗原基因 转基因植物 农杆菌 植物生物反应器 plant bioreactors vaccine vector Agrobacterium tumefaciens
分类号 TQ464 [化学工程—制药化工] TQ460.38 [化学工程—制药化工]
  • 相关文献

参考文献8

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共引文献9

同被引文献30

  • 1刘铀,毕英佐,马静云,曹永长.口蹄疫病毒VP1植物表达载体的构建[J].湛江海洋大学学报,2004,24(4):59-62. 被引量：1
  • 2余祖华,王红宁.用植物生物反应器研制基因工程疫苗的进展[J].生物技术,2004,14(4):78-80. 被引量：10
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引证文献3

二级引证文献3

吉林农业大学学报
2003年 第3期

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