摘要
目的 观察p2 7基因表达对胃癌SGC790 1细胞端粒酶活性及细胞周期的影响。方法 采用腺病毒介导的方法将p2 7kip1cDNA导入胃癌SGC790 1细胞中 ,应用TRAP -ELISA法检测细胞端粒酶活性的变化 ,流式细胞仪分析细胞周期的变化。结果 转染了 p2 7kip1cDNA的细胞端粒酶活性显著下降 ,G1期细胞比率显著增高 ,细胞增殖指数显著降低。结论 外源性 p2 7基因的表达可使SGC790 1细胞生长停滞于G1期 ,端粒酶活性下降 ,Ad -p2
Objective To investigate the effect of p27kip1cDNA on the telomerase activity and cell cycle of SGC7901 cells.Methods A replication-deficient adenovirus vector encoding p27kip1(Ad-p27kip1) was transduced into human gastric carcinoma cell lines SGC7901 in vitro.Telomerase activity of transduced cells was detected by TRAP-ELISA(Telomeric Repeat Amplification Protocol and enzyme-linked immunosorbant assay).Cell cycle was determined using flow cytometry(FCM) analysis.Results Telomerase activity of Ad-p27kip1 transduced group decreased obviously compared with the control group.FCM data showed that the percent of cells at G1 phase was increased and cell proliferation index decreased significantly.Conclusion Exogenous p27 gene expression blocks cell cycle at G1 phase and reduces cell telomerase activity.Ad-p27kip1 has potential therapeutic effect on gastric cancer.
出处
《实用癌症杂志》
2003年第3期253-256,共4页
The Practical Journal of Cancer
