摘要
试验表明 :火炬姜组培快繁以MS +6 BA 2 0mg/L +NAA 0 1mg/L +CW 10 %作为增殖培养基可以获得理想的效果 ,增殖倍数可达 4 3倍 ;以双芽为外植体在该培养基上经 30d培养可增殖 5 4倍 ;在不含任何激素的 1/ 2MS +0 15 %活性炭培养基上即可形成较发达的根系 ,生根率达 10 0 %;试管苗移栽对基质要求不严 ,移栽到疏松透气、富含有机质的表土中 ,成活率高达 10 0 %,而且植株生长良好。
The research result of the in vitro propagation of the Phaeomeria magnifica is presented in this paper.The results showed that:Using MS+6-BA 2.0 mg/L+NAA 0.1 mg/L+CW 10% as proliferation medium,an optimal proliferation rate was obtained,the proliferation rate could reach 4.3 times;Using double shoots as explants,after 30 days of culture in this medium,it could amplify 5.4 times.The root formation of Phaeomeria magnifica tissue culture shoots had a low demand for exotic hormones,even in a medium of no hormones it could still form a good root system,the rooting rate could reach 100%.The transplantation of Phaeomeria magnifica tissue culture shoots was simple and feasible.There was no strict demand for matrix,when the plantlets were transplanted to superficial soil,the survival rate could reach 100%,and the plants grew well.
出处
《园艺学报》
CAS
CSCD
北大核心
2003年第2期183-186,共4页
Acta Horticulturae Sinica
关键词
火炬姜
离体快繁技术
丛生芽
试管苗
菲律宾蜡花
Phaeomeria magnifica
Adventitious shoot
Rapid propagation via tissue culture
