摘要
目的 探讨阿托伐他汀对间质纤维化大鼠肾间质巨噬细胞积聚的影响及其可能的机制。方法 2 1只大鼠随机分为假手术、单侧输尿管梗阻 (UUO)、UUO +阿托伐他汀治疗组 ,分别用免疫组化染色检测巨噬特异性抗原CD68和巨噬细胞集落刺激因子 (M -CSF)以确定肾间质巨噬细胞数和M -CSF的量 ,并常规查各组血脂。结果 单侧输尿管梗阻第 10d肾间质巨噬细胞积聚和M -CSF表达明显增加 ,阿托伐他汀治疗后二者均明显减少。模型组和治疗组巨噬细胞积聚程度与小管间质M -CSF表达呈正相关。各组血脂无显著性差异。结论 阿托伐他汀可显著减少肾间质巨噬细胞积聚程度 ,这可能与其下调小管间质M -CSF表达有关。
Objective To investigate the effect of artorvastatin on macrophage accumulation in tubulointerstitium of unilateral ureteral obstructive (UUO) nephropathy and its possible mechanism.Methods Twenty-one rats were randomly divided into three groups: sham-operatipn, UUO, UUO+ artorvastatin. Immunohistochemistry staining of CD68 and M-CSF was used to define the macrophage accumulation and expression of interstitial M-CSF. Lipid profile in these groups was also determined. Results CD68 + cells and M-CSF expression were significantly increased at day 10 after UUO operation, this kind of CD68 + cell accumulation and M-CSF expression up-regulation were ameliorated by artorvastatin treatment. In UUO and atorvastatin treated groups, the number of macrophage was positively correlated with tubulointerstitial M-CSF expression. There was no significant difference about serum lipid among the three groups. Conclusion Atorvastatin can reduce interstitial macrophage accumulation in UUO nephropathy. This therapeutic effect might relate to down-regulation of tubulointerstitial M-CSF expression.
出处
《中国医师杂志》
CAS
2003年第4期460-462,共3页
Journal of Chinese Physician
