摘要
目的 :研究延时脱钙和骨的几何形状对骨基质成骨能力的影响。方法 :切取鼠股骨干分成 5组 ,骨干 2 4h组、骨干 48h组 (对照组 )、骨干 48B组和骨干 72h组 ,分别用 0 .6NHC1脱钙 2 4、48或 72h。将颗粒 48B组的骨碾磨成12 5～ 80 0 μm的颗粒。 5 0只Wistar鼠作为受体。术后 6周取标本测定重量、干燥后的重量、钙含量、碱性磷酸酶含量和组织学观察。结果 :颗粒 48H组的干燥重量高于控制组 ,二者有统计学差别。结论 :骨延时脱钙不影响其成骨能力。 12 5～ 80 0 μm的颗粒状鼠骨基质成骨能力优于块状骨基质。
Objective:To investigate the effects of prolonging demineralization and geometric architecture of bone matrix on the capacity of osteogenesis.Methods:The rats' femurs were divided into five groups.The diaphyses of Seg24h,Seg48h(control group),Seg48B and Seg 72h groups were decalcified separately with 0.6N HCl for 24,48 or 72 hours.The bone in Pow48B group were pulverized into 125-800 micron of bone.50 Wistar rats acted as a receptors.Evaluations were assessed by the explanted weight ratio,calcium and alkaline phosphatase contents,and histological observation 6 weeks postoperatively.Results:The dried weight in the pow 48H group was significant greater than that in the control group.Conclusion:The prolonging demineralization of bone does not influence the matrix induced osteogenesis.The rats bone pulverized in the particle size of 125 800 microns has more newly formed bone than did in the segmental bone.
出处
《中国矫形外科杂志》
CAS
CSCD
2003年第2期108-109,共2页
Orthopedic Journal of China
关键词
异体骨
脱钙
几何形状
成骨能力
Bone allografts
Demineralization
Geometric architecture
Osteogenesis
