摘要
目的 :探讨胰岛素样生长因子Ⅱ (IGF Ⅱ )表达水平与结直肠癌细胞增殖、凋亡 (程序性死亡 )的关系及其意义。 方法 :结直肠腺癌患者 36例 ,术前纤维结肠镜直视下采集癌和正常结直肠粘膜组织标本。以RT PCR法检测IGF ⅡmRNA。S P法检测IGF Ⅱ ,多媒体图文分析系统定量阳性染色区灰度值。S P法检测增殖细胞核抗原(PCNA) ,计算PCNA标记指数。TUNEL法检测凋亡细胞 ,计算凋亡指数。 结果 :结直肠癌组织中IGF ⅡmR NA表达值 (0 .6 5± 0 .17)显著高于正常粘膜 (0 .4 7± 0 .2 2 )。IGF Ⅱ蛋白水平 (2 0 .2 9± 8.0 4 )亦显著高于正常粘膜(13.81± 5 .86 ) ,二者呈良好线性相关 (r =0 .74 74 ,P <0 .0 1)。IGF Ⅱ灰度值与PCNA标记指数、凋亡指数高低亦显著相关。 结论 :结直肠癌组织中IGF Ⅱ高表达能影响癌细胞的生物学特性 。
Objectives: To investigate the relationship of insulin like growth factor Ⅱ(IGF Ⅱ), proliferating cell nuclear antigen(PCNA) and apoptosis in colorectal cancer. Methods: Thirty six colorectal cancer patients were enrolled. In colonoscopic bioptic specimens, IGF Ⅱ mRNA was detected by RT PCR, the expression of IGF Ⅱ and PCNA were detected immunohistochemically, while TUNEL technique was adopted to detect apoptosis. Results: The expression level of IGF Ⅱ mRNA and IGF Ⅱ were significantly higher in colorectal cancer than in normal colorectal mucosa. A positive correlation was found between IGF Ⅱ expression and PCNA labeling index, so as IGF Ⅱ expression and apoptotic index. Conclusions: Expression level of IGF Ⅱ might be a new prognostic predictor for colorectal cancer.
出处
《医学研究生学报》
CAS
2002年第6期502-504,共3页
Journal of Medical Postgraduates
