摘要
目的探讨Toll样受体4(TLR4)/髓样分化因子88(MyD88)信号通路相关蛋白与结直肠癌患者临床特征和预后的关系。方法选择2019年1月至2022年1月驻马店市中心医院收治的结直肠癌患者95例为研究对象。患者入院后均接受规范化综合治疗。采用流式细胞仪检测患者治疗前TLR4/MyD88信号通路相关蛋白TLR4、MyD88、肿瘤坏死因子受体相关因子-4(TRAF-4)、核因子-κB(NF-κB)水平。根据生存情况将患者分为存活组(n=58)和死亡组(n=37)。收集所有患者的临床资料,采用Spearman相关性分析TLR4/MyD88信号通路相关蛋白水平与患者临床特征的关系;采用单因素分析和logistic回归分析TLR4/MyD88信号通路相关蛋白与结直肠癌患者预后的关系。结果不同肿瘤部位患者血清TLR4、MyD88、TRAF-4、NF-κB表达水平比较差异无统计学意义(P>0.05);不同浸润深度、淋巴结转移情况、远处转移情况、TNM分期、分化程度患者血清TLR4、MyD88、TRAF-4、NF-κB表达水平比较差异均有统计学意义(P<0.05)。Spearman相关性分析结果显示,肿瘤部位与TLR4、MyD88、TRAF-4、NF-κB表达水平无显著相关性(P>0.05);浸润深度、淋巴结转移、远处转移、TNM分期与TLR4、MyD88、TRAF-4、NF-κB表达水平呈正相关(P<0.05);分化程度与TLR4、MyD88、TRAF-4、NF-κB表达水平呈负相关(P<0.05)。单因素分析和logistic回归分析结果显示,TLR4(高)、MyD88(高)、TRAF-4(高)、NF-κB(高)、浸润深度(T_(4))、淋巴结转移(N_(2))、远处转移(M 1)、TNM分期(Ⅳ期)、分化程度(低分化)是影响结直肠癌预后的危险因素(P<0.05)。结论TLR4/MyD88信号通路相关蛋白在结直肠癌患者体内呈高表达,且与结直肠癌患者临床特征中浸润深度、淋巴结转移、远处转移、TNM分期、分化程度呈显著相关性,同时对结直肠癌患者预后有较高预测价值。
Objective To explore the relationship of Toll-like receptor 4(TLR4)/myeloid differentiation primary response protein 88(MyD88)signaling pathway related proteins with the clinical characteristics and prognosis of patients with colorectal cancer.Methods Ninety-five patients with colorectal cancer admitted to Zhumadian Central Hospital from January 2019 to January 2022 were included as the study subjects.All patients received standardized comprehensive treatment after admission,and the levels of TLR4/MyD88 signaling pathway related proteins including TLR4,MyD88,tumor necrosis factor receptor-associated factor-4(TRAF-4)and nuclear factor(NF)-κB were detected by a flow cytometer before treatment.The patients were divided by their survival status into a survival group(n=58)and a death group(n=37).The clinical data of the patients were collected and the Spearman correlation method was used to analyze the relationship between TLR4/MyD88 signaling pathway related proteins and the clinical characteristics of patients.Univariate and logistic regression analysis were made to analyze the relationship between TLR4/MyD88 signaling pathway related proteins and the prognosis of colorectal cancer patients.Results There were no significant differences in serum TLR4,MyD88,TRAF-4 and NF-κB among patients with tumors at different sites(P>0.05).There were significant differences in serum TLR4,MyD88,TRAF-4 and NF-κB among patients with different depths of invasion,lymph node metastasis,distant metastasis,TNM stages and differentiation grades(P<0.05).Spearman correlation analysis showed no correlation of the expression levels of serum TLR4,MyD88,TRAF-4 and NF-κB with the tumor site(P>0.05).The expression levels of serum TLR4,MyD88,TRAF-4 and NF-κB were correlated positively with the depth of invasion,lymph node metastasis,distant metastasis and TNM stage,and negatively with differentiation grades(P<0.05).Univariate and logistic regression analysis showed that high TLR4,MyD88,TRAF-4 and NF-κB levels,an invasion depth of T_(4),lymph node metastasis of N_(2),distant metastasis of M1,TNM stageⅣ,and low differentiation were risk factors of poor prognosis of patients with colorectal cancer(P<0.05).Conclusion TLR4/MyD88 signaling pathway related proteins are highly expressed in patients with colorectal cancer,and significantly correlated with the clinical characteristics of colorectal cancer,such as depth of invasion,lymph node metastasis,distant metastasis,TNM stage,and differentiation grade.They have high predictive value for the prognosis of patients with colorectal cancer.
作者
刘海龙
谭首海
于秀芝
李海超
张辰宇
LIU Hailong;TAN Shouhai;YU Xiuzhi;LI Haichao;ZHANG Chenyu(Department of Anorectal,Zhumadian Central Hospital,Zhumadian 463000,Henan Province,China)
出处
《新乡医学院学报》
2025年第4期288-293,共6页
Journal of Xinxiang Medical University
关键词
结直肠癌
Toll样受体4/髓样分化因子88信号通路
临床特征
colorectal cancer
Toll-like receptor 4/myeloid differentiation primary response protein 88 signaling pathway
clinical characteristics
