摘要
彩色青稞和彩色小麦是一类特殊的、珍贵的种质资源,不同颜色籽粒中的营养物质成分和含量均不同。本研究基于LC-MS对2个彩色青稞品种鄂黑稞720135(EH720135)、广州蓝稞(GZL)和2个彩色小麦品种中科紫麦(ZKZM)、农大4218紫(ND4218)授粉后不同天数的籽粒代谢组进行了分析和比较。结果表明:在4个材料不同发育时期的籽粒中,共检测到936种代谢物,包含379种已知物质和557种未知物。聚类分析表明,不同发育时期的籽粒中代谢物存在明显差异,各种代谢物的动态积累模式也不尽相同。对4个材料成熟籽粒中代谢物进行比较分析,在彩色青稞和彩色小麦之间共鉴定出687种差异代谢物,其中,206种在2个彩色青稞和2个彩色小麦之间均存在显著差异。此外,还鉴定到308种和277种代谢物分别在2个彩色小麦之间和2个彩色青稞之间存在显著差异。进一步分析表明,成熟青稞籽粒中的儿茶素、麦黄酮衍生物、金圣草黄素等黄酮类相关代谢物含量显著高于彩色小麦,它们可能为青稞特异的功能性成分。本研究有助于全面了解彩色青稞和彩色小麦籽粒代谢物的差异,并为彩色青稞和彩色小麦功能性食品研发提供理论依据。
Colored hulless barley and wheat are valuable and unique germplasm resources,with nutrient composition and content varying among grains of different colors.In this study,metabolomic analysis based on LC-MS was applied to grains from two colored hulless barley varieties and two wheat varieties at different days after pollination,including Ehei 720135(EH720135),Guangzhou Blue Barley(GZL),Zhongke Zimai(ZKZM),and Nongda 4218 Zi(ND4218).A total of 936 metabolites were detected across the 23 samples,comprising 379 known substances and 557 unknowns.Cluster analysis and PCA revealed significant differences in the metabolite profiles of grains at different developmental stages.Most metabolites exhibited dynamic changes throughout grain development.In total,687 metabolites showed differential abundances between the mature seeds of the four varieties,of which 206 were significantly different between the two hulless barley varieties and the two wheat varieties.Furthermore,308 differential metabolites were identified between the two-colored wheat varieties,and 277 between the two colored hulless barley varieties.Further analysis revealed that metabolites related to flavonoids,such as catechins,flavonoid derivatives,and chrysoeriol,were significantly more abundant in mature hulless barley grains compared to colored wheat grains,suggesting that these may be specific functional components of hulless barley.This study not only enhances our understanding of the metabolic differences between hulless barley and wheat,but also provides a theoretical foundation for the development of functional foods using colored hulless barley and colored wheat.
作者
程红娜
秦丹丹
许甫超
徐晴
彭严春
孙龙清
徐乐
郭英
杨新泉
徐得泽
董静
CHENG Hong-Na;QIN Dan-Dan;XU Fu-Chao;XU Qing;PENG Yan-Chun;SUN Long-Qing;XULe;GUO Ying;YANG Xin-Quan;XU De-Ze;DONG Jing(College of Agriculture,Yangtze University,Jingzhou 434023,Hubei,China;Key Laboratory of Crop Molecular Breeding,Ministry of Agriculture and Rural Affairs/Hubei Key Laboratory of Genetic Improvement and Germplasm Innovation of Food Crops/Hubei Hongshan Laboratory/Institute of Food Crops,Hubei Academy of Agricultural Sciences,Wuhan 430064,Hubei,China;Seed Laboratory of Yazhou Bay,Sanya 572024,Hainan,China)
出处
《作物学报》
北大核心
2025年第4期932-942,共11页
Acta Agronomica Sinica
基金
财政部和农业农村部国家现代农业产业技术体系建设专项(CARS-05)
优质青贮大麦新品种选育及冬闲田应用关键技术研发(2023BBB100)
功能作物新种质创制及其食品研发和功效评价(2022ZZCX006)资助。
