摘要
目的探讨不同组织来源生物补片重建阴道的效果。方法以巴马小型猪为动物模型,手术切除阴道,构建阴道缺失的大动物模型,并以猪小肠黏膜的脱细胞基质(SIS)和猪膀胱的脱细胞基质(ABM)重建小型猪阴道组织。于术后3个月手术切除重建后的阴道组织,通过组织病理学染色和组织浴槽验证重建阴道组织结构和功能。结果重建术后3个月,SIS和ABM可在解剖学上重建阴道。通过组织学和功能学比较,SIS和ABM组织结构与正常阴道一致,并可表达阴道的组织特异性分子标记物CK14、α-actin、HSP47、ER、CD31。但SIS重建组的阴道上皮细胞层数较ABM重建组少,表明ABM重建效果可能优于SIS。同时,PAS染色及组织浴槽实验也证实,重建的阴道上皮细胞可合成糖原,且相应药物可引起舒缩反应。这些结果表明,SIS和ABM能够在大体、组织结构和功能上重建阴道。结论巴马小型猪可作为阴道重建再生的大动物模型。SIS和ABM能够达到解剖学、组织学和功能学重建,但ABM的重建效果可能优于SIS。
Objective To evaluate the effectiveness of biological patches derived from different tissue sources for vaginal reconstruction.Methods Large animal models of vaginal agenesis were established using Bama miniature pigs through surgical removal of vaginal tissue.Small intestinal submucosa(SIS)and acellular bladder matrix(ABM)were used for vaginal reconstruction.Three months postoperatively,the reconstructed vaginal tissues were surgically removed and evaluated for tissue structure and function using histopathological staining and organ bath assays.Results Three months after reconstruction,both SIS and ABM achieved anatomical reconstruction of the vagina.Histological and functional analyses revealed that the tissue structures of SIS-and ABM-reconstructed vaginas resembled normal vaginal tissue and expressed vaginal-specific molecular markers,including CK14,α-actin,HSP47,ER,and CD31.However,the SIS-reconstructed group exhibited a thinner vaginal epithelial layer compared to the ABM-reconstructed group,indicating that ABM may provide better reconstruction outcomes.Additionally,PAS staining and organ bath assays confirmed that the reconstructed vaginal epithelial cells were capable of synthesizing glycogen and exhibited contractile and relaxant responses to corresponding drugs.These results indicated that both SIS and ABM could achieve vaginal reconstruction in terms of gross anatomy,tissue structure,and function.Conclusion Bama miniature pigs can serve as suitable large animal models for vaginal tissue regeneration and reconstruction.Both SIS and ABM can achieve anatomical,histological,and functional vaginal reconstruction,with ABM potentially offering better outcomes than SIS.
作者
张琳
田彦鹏
肖艳来
李仲康
刘义彬
张明乐
张敬坤
孟丽
戴建武
黄向华
Zhang lin;Tian Yanpeng;Xiao Yanlai;Li Zhongkang;Liu Yibin;Zhang Mingle;Zhang Jingkun;Meng Li;Dai Jianwu;Huang Xianghua(The Second Hospital of Hebei Medical University,Shijiazhuang 050000,Hebei,China;The First Affiliated Hospital of Zhengzhou University,Zhengzhou 450052,Henan,China;The Third Hospital of Hebei Medical University,Shijiazhuang 050000,Hebei,China;Institute of Genetics and Developmental Biology,Chinese Academy of Sciences,Beijing 100101,China)
出处
《生物医学转化》
2025年第1期87-92,共6页
Biomedical Transformation
基金
国家自然科学基金面上项目(8167060210)
河北中央引导地方科技发展资金项目(236Z7756G)
河南省医学科技攻关计划联合共建项目(LHGJ20220359)。
关键词
MRKH综合征
生物补片
巴马小型猪
阴道重建
组织工程
MRKH syndrome
Biological patches
Bama miniature pigs
Vaginal reconstruction
Tissue engineering
