摘要
目的探讨结直肠息肉患者的肠道菌群结构和丰度与结直肠组织PH结构域和富含亮氨酸的重复蛋白磷酸酶(PHLPP)、胰岛素样生长因子结合蛋白5(IGFBP5)相对表达水平的相关性。方法回顾性分析2021年1月至2023年12月上海中医药大学附属曙光医院内镜中心诊治的82例结直肠息肉患者(息肉组)和76例肠镜检查阴性者(对照组)的临床资料。比较2组结直肠组织PHLPP、IGFBP5相对表达水平、肠道菌群结构(包括Chao指数、Ace指数、Simpson指数)和肠道菌群(拟杆菌门、厚壁菌门、梭杆菌门、放线菌门、变形菌门和厌氧芽孢杆菌属、双歧杆菌属、埃希菌属、不动杆菌属、拟杆菌属)丰度。采用Pearson相关分析肠道菌群结构和丰度与结直肠组织PHLPP、IGFBP5相对表达水平的相关性。结果息肉组PHLPP相对表达水平低于对照组(P<0.05),IGFBP5相对表达水平高于对照组(P<0.05)。息肉组Chao指数、Ace指数均高于对照组(P<0.05),但息肉组和对照组Simpson指数比较,差异无统计学意义(P>0.05)。息肉组拟杆菌门、梭杆菌门丰度均低于对照组(P<0.05),而厚壁菌门、放线菌门、变形菌门丰度均高于对照组(P<0.05)。息肉组埃希菌属、拟杆菌属丰度均低于对照组(P<0.05),而厌氧芽孢杆菌属、双歧杆菌属、不动杆菌属丰度均高于对照组(P<0.05)。Pearson相关分析结果显示:息肉组患者Chao指数、Ace指数及厚壁菌门、放线菌门、变形菌门、厌氧芽孢杆菌属、双歧杆菌属、不动杆菌属丰度均与结直肠组织PHLPP相对表达水平呈负相关(P<0.05),与结直肠组织IGFBP5相对表达水平呈正相关(P<0.05);息肉组患者拟杆菌门、梭杆菌门、埃希菌属、拟杆菌属丰度均与结直肠组织PHLPP相对表达水平呈正相关(P<0.05),与结直肠组织IGFBP5相对表达水平呈负相关(P<0.05)。结论结直肠息肉患者肠道菌群结构和肠道菌群丰度均发生异常变化,且肠道菌群结构和丰度与结直肠组织PHLPP、IGFBP5相对表达水平有关。
Objective To explore the relationship of the structure and abundance of intestinal fl ora in colorectal polyps patients with the relative expression levels of PH domain and Leucine rich repeat protein phosphatases(PHLPP)and i nsulin-like growth factor-binding protein 5(IGFBP5)in colorectal tissue.Methods Clinical data of 82 patients with colorectal polyposis(polyp group)and 76 colonoscopy-negative patients(control group)diagnosed and treated at the Endoscopy Center of Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine from January 2021 to December 2023 were retrospectively analyzed.The relative expression levels of PHLPP and IGFBP5 in colorectal tissues,intestinal flora structures(including Chao index,Ace index,Simpson index)and intestinal flora(Bacteroidetes,Firmicutes,Fusobacteria,Actinobacteria,Proteobacteria and Anaerobic spore-forming bacillus,Bifidobacterium,Escherichia,Acinetobacter,Bacteroides genera)abundances were compared between the two groups.Pearson correlation was used to analyze the relationship of intestinal flora structures and abundances with the relative expression levels of PHLPP and IGFBP5 in colorectal tissue.Results The relative expression level of PHLPP in the polyp group was lower than that in the control group(P<0.05),and the relative expression level of IGFBP5 was higher than that in the control group(P<0.05).The Chao index and Ace index of the polyp group were higher than those of the control group(P<0.05),but there was no significant difference in Simpson index between the polyp group and the control group(P>0.05).The abundances of Bacteroidetes and Fusobacteria in the polyp group were lower than those in the control group(P<0.05),and the abundances of Firmicutes,Actinobacteria,Proteobacteria in the polyp group were higher than those in the control group(P<0.05).The abundances of Escherichia genera and Bacteroides genera in the polyp group were lower than those in the control group(P<0.05),and the abundances of Anaerobic spore-forming bacillus genera,Bifidobacterium genera and Acinetobacter genera in the polyp group were higher than those in the control group(P<0.05).Pearson correlation analysis results showed that the Chao index,Ace index and the abundances of Firmicutes,Actinobacteria,Proteobacteria,Anaerobic spore-forming bacillus genera,Bifidobacterium genera and Acinetobacter genera in the polyp group were negatively correlated with the relative expression level of PHLPP in colorectal tissue(P<0.05),and positively correlated with the relative expression level of GFBP5 in colorectal tissue(P<0.05).The abundances of Bacteroidetes,Fusobacteria,Escherichia genera and Bacteroides genera in the polyp group were positively correlated with the relative expression level of PHLPP in colorectal tissue(P<0.05),and negatively correlated with the relative expression level of GFBP5 in colorectal tissue(P<0.05).Conclusion The intestinal flora structures and abundances of patients with colorectal polyp are abnormally changed,and the intestinal flora structures and abundances are related to the expression levels of PHLPP and IGFBP5 in colorectal tissue.
作者
杨姗莹
庄羽骁
郭丽坤
岑戎
YANG Shanying;ZHUANG Yuxiao;GUO Likun;CEN Rong(Endoscopy Center,Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine,Shanghai 200021,China)
出处
《检验医学与临床》
2025年第6期737-741,共5页
Laboratory Medicine and Clinic
基金
上海浦东新区卫生健康委员会卫生计生科研项目(PW2020D-4)。
