摘要
试验旨在增强植物乳植杆菌(Lactiplantibacillum plantarum)的抗逆性,提高其经胃肠道后的存活率。试验以柑橘纤维素和柑橘果胶为原料,对植物乳植杆菌B5 (L. plantarum B5)进行包埋,构建复合乳酸菌微胶囊,通过测定包埋率、结构表征、控释试验、耐胆盐及储藏稳定性试验,对复合微胶囊进行综合评估。结果显示,微胶囊对L. plantarum B5具有良好的包埋效果,当柑橘纤维素和柑橘果胶的配比为1∶3时(CL13微胶囊),包埋率最高达到95.82%。傅里叶红外光谱图表明,纤维素和果胶中的羧基与钙离子通过桥连配位形成微胶囊。扫描电镜图像显示,菌体成功包埋于微胶囊的内部及表面。所有微胶囊均具备良好的控释性能,CL13微胶囊的控释效果显著高于其他配比的微胶囊(P<0.05),在模拟肠液中的释放率达到90.28%。微胶囊能够显著提高L. plantarum B5在胆盐和低温环境下的存活率(P<0.05),微胶囊稳定性良好。研究表明,柑橘纤维-柑橘果胶微胶囊能够有效保护L. plantarum B5顺利到达肠道,在宿主体内最大限度地发挥益生作用。
The study aimed to enhance the stress resistance of Lactiplantibacillumplantarumand improve its survival rate after gastrointestinal passage.The experiment used citrus cellulose and citrus pectin as raw materials to encapsulate LactiplantibacillusplantarumB5(L.plantarumB5)to construct composite probiotic microcapsules,and the microcapsules were comprehensively evaluated through the measurement of encapsulation rate,structural characterization,controlled-release test,bile-salt resistance and storage stability test.The results showed that the microcapsules show excellent encapsulation of L.plantarum B5,with the highest encapsulation rate of 95.82%when the ratio of citrus cellulose and citrus pectin was 1∶3(CL13 microcapsules).Fourier infrared spectrograms show that carboxyl groups in cellulose and pectin form microcapsules by bridging coordination with calcium ions.Scanning electron microscopy images demonstrate the successful encapsulation of the bacterium in the interior and on the surface of the microcapsules.All microcapsules possess excellent controlled release properties,and CL13 microcapsules show significantly higher controlled release than the other formulations(P<0.05),with a release rate of 90.28%in simulated intestinal fluid.Microcapsules could significantly increase the survival of L.plantarumB5 in bile salts and at low temperatures(P<0.05),and microcapsules are well stabilized.The study indicates that citrus cellulose-citrus pectin microcapsules are effective in protecting L.plantarumB5 from reaching the intestinal tract,allowing them to maximize their beneficial effects in the host.
作者
王少杨
李子院
陈红联
覃黎娜
姜铁民
李海云
蒋季岑
WANG Shaoyang;LI Ziyuan;CHEN Honglian;QIN Lina;JIANG Tiemin;LI Haiyun;JIANG Jicen
出处
《饲料研究》
北大核心
2025年第2期127-133,共7页
Feed Research
基金
中央引导地方科技发展专项资金(项目编号:桂科ZY22096025)
桂林市创新平台和人才计划(项目编号:20220124-4)。
关键词
植物乳植杆菌
柑橘纤维素
柑橘果胶
包埋率
控释性能
Lactiplantibacillumplantarum
citrus cellulose
citrus pectin
embedding rate
controlled release performance
