摘要
目的:探究不同工艺制备的纳晶纤维素(cellulose nanocrystals,CNC)口服安全性及肠道菌群调节作用。方法:将小鼠按雌、雄分6组,每组12只,即空白雌(Control_F)、雄(Control_M)小鼠组,硫酸水解CNC雌(S-CNC_F)、雄(S-CNC_M)小鼠组和磷酸水解CNC雌(P-CNC_F)、雄(P-CNC_M)小鼠组。考察灌胃后小鼠的活动行为、体质量、体成分、脏器系数、血清生物学指标及脏器苏木精-伊红(hematoxylin-eosin,HE)染色病理情况。采集粪便进行16S rDNA高通量测序,分析S-CNC和P-CNC对小鼠肠道菌群的影响。结果:与空白组小鼠相比,S-CNC和P-CNC组小鼠在灌胃后活动行为、体质量、脂肪、液体、瘦肉成分及脏器系数均无显著性差异(P>0.05)。灌胃S-CNC及P-CNC对小鼠血清中丙氨酸氨基转移酶、天门冬氨酸氨基转移酶、尿酸和血尿素氮含量无影响。HE染色病理结果表明,S-CNC组和P-CNC组小鼠主要脏器组织形态结构均未见异常。灌胃S-CNC和P-CNC改变了正常小鼠肠道菌群的丰富度和均匀度,并且对小鼠的肠道菌群组成在门、科及属水平上有一定的改变。在门水平上,S-CNC增加了雌性小鼠厚壁菌门的相对丰度,同时也降低了雄性小鼠厚壁菌门和雌、雄小鼠拟杆菌门相对丰度。P-CNC增加了雌性小鼠厚壁菌门和雄性小鼠拟杆菌门的相对丰度,而降低了雄性小鼠厚壁菌门和雌性小鼠拟杆菌门相对丰度。在科水平上,灌胃S-CNC和P-CNC后,雌、雄性小鼠肠道中阿克曼氏菌科和动球菌科相对丰度增加。属水平分析显示,灌胃S-CNC后,雄性小鼠肠道中阿克曼菌属和梭菌属相对丰度增加;雌性小鼠肠道中不动杆菌属相对丰度增加。灌胃P-CNC后,雌性小鼠肠道中库特菌属和阿克曼菌属相对丰度增加,而雄性小鼠假丝酵母菌属相对丰度降低。结论:不同工艺提取的CNC在小鼠体内有良好安全性,并且S-CNC和P-CNC对小鼠肠道菌群有一定的调节作用。
Objective:This study was conducted to investigate the oral safety and gut microbiota regulatory effect of cellulose nanocrystals(CNC)prepared by sulfuric acid hydrolysis(S-CNC)or phosphoric acid hydrolysis(P-CNC).Methods:Mice were divided into three groups(24 mice per group,half from each gender):control,S-CNC and P-CNC.The changes in activity behavior,body mass,body composition,organ indexes and serum biochemical indexes were examined after gavage,and pathomorphological changes in various internal organs were observed by hematoxylin-eosin(HE)staining.Feces were collected for 16S rDNA gene high-throughput sequencing to analyze the effects of S-CNC and P-CNC on the gut microbiota of mice.Results:Compared with the control group,no significant differences were observed in activity behavior,body mass,fat,fluid,lean components or organ indexes in the S-CNC and P-CNC groups(P>0.05).The serum levels of alanine aminotransferase(ALT),aspartate aminotransferase(AST),uric acid(UA)and urea were unaffected by S-CNC and P-CNC administration.HE staining showed that neither CNC treatment exhibited morphological abnormalities in the major organs.S-CNC and P-CNC changed the abundance and uniformity of gut microbiota in normal mice as well as the composition ofgut microbiota at the phylum, family and genus levels. S-CNC increased the relative abundance of Firmicutes in female mice,but decreased the relative abundance of Firmicutes in male mice and the relative abundance of Bacteroidetes in both maleand female mice. P-CNC increased the relative abundance of Firmicutes in female mice and Bacteroidetes in male mice, butdecreased the relative abundance of Firmicutes in male mice and the relative abundance of Bacteroidetes in female mice. Atthe family level, the relative abundance of Akkermansiaceae and Planococcaceae in the intestinal tracts of female and malemice increased after administration of S-CNC and P-CNC. Genus level analysis showed that S-CNC administration increasedthe relative abundance of Akkermansia and Faecalibaculum in male mice and the relative abundance of Acinetobacter in thegut of female mice. After administration of P-CNC, the relative abundance of Kurthia and Akkermansia increased in femalemice, while the relative abundance of Candida decreased in male mice. Conclusion: S-CNC and P-CNC are safe for mice toeat. Moreover, both of them have a regulatory effect on the gut microbiota in mice.
作者
明良山
肖楠
刘澳
萧子健
刘红宁
李哲
MING Liangshan;XIAO Nan;LIU Ao;XIAO Zijian;LIU Hongning;LI Zhe(Key Laboratory of Modern Preparation of Traditional Chinese Medicine,Ministry of Education,Institute for Advanced Study,Jiangxi University of Chinese Medicine,Nanchang 330004,China)
出处
《食品科学》
北大核心
2025年第6期142-155,共14页
Food Science
基金
国家自然科学基金青年科学基金项目(82204630)
国家自然科学基金地区科学基金项目(82460775)
江西省自然科学基金面上项目(20232BAB206166)
江西中医药大学博士启动项目(2022BSZR003,2021BSZR015)
江西省2024年度研究生创新专项(YC2024-S751)。
关键词
纳晶纤维素
安全性
肠道菌群
硫酸水解
磷酸水解
cellulose nanocrystals
safety
gut microbiota
sulfuric acid hydrolysis
phosphoric acid hydrolysis
