摘要
目的通过建立反复自然流产(RSA)小鼠模型,研究血管内皮损伤相关circRNAs与复发性流产的关联性及其调控机制。方法选取8只正常妊娠模型的孕鼠设为对照组,将8只复发性流产模型的孕鼠设为实验组。基于VEGF及生物信息学构建ceRNA网络,对miRNA-VEGF和circRNAs调控网格在RSA中的作用和机制进行分析,对比两组小鼠circRNA-miRNA的相对量,分析目标circRNA-miRNA及影响因素的非条件Logistic回归结果。结果目标circRNA-miRNA在两组之间的相对量表达,差异具有统计学意义(P<0.05),miR-10a-5p在实验组中低表达,而circ-0014243、circ-0126991在实验组中高表达。通过对血管内皮损伤相关circRNA与miRNA进行研究分析,经过相对表达量的分析发现,miR-497-5p、miR-10a-5p、circ-0014243、circ-0126991比较,差异有统计学意义(P<0.05),而circ-0041000比较,差异无统计学意义(P>0.05)。结论基于构建ceRNA调控网络、RT-PCR及细胞及动物层面转染circRNA及miRNA进行验证,从而明确VEGF在RSA中的潜在机制,以此为临床治疗提供潜在靶标。
Objective To establish a mouse model of recurrent spontaneous abortion(RSA)and to study the correlation of circRNAs associated with vascular endothelial injury and recurrent abortion and its regulatory mechanism.Methods 8 pregnant mice with normal pregnancy model were selected as the control group,and 8 pregnant mice with recurrent abortion model were selected as the experimental group.The ceRNA network was constructed based on VEGF and bioinformatics,and the roles and mechanisms of miRNA-VEGF and circRNAs regulatory grid in RSA were analyzed,and the relative amounts of circRNA-miRNA in the two groups of mice were compared.Non-conditional Logistic regression results of target circRNA-miRNA and influencing factors were analyzed.Results The relative expression of the target circRNA-miRNA between the two groups was statistically significant differences(P<0.05).The expression of miR-10a-5p was low in the experimental group,while circ-0014243 and circ-0126991 were high in the experimental group.Through the study and analysis of circRNA and miRNA associated to vascular endothelial injury,the relative expression levels of miR-497-5p,miR-10a-5p,circ-0014243,circ-0126991 were compared,and the differences were statistically significant(P<0.05),while there was no significant difference in circ-0041000(P>0.05).Conclusion Based on the construction of ceRNA regulatory network,RT-PCR,and transfection of circRNA and miRNA at the cell and animal levels,the potential mechanism of VEGF in RSA was identified,so as to provide potential targets for clinical treatment.
作者
林丽慧
闫志强
任青
曾思衡
Lin Lihui;Yan Zhiqiang;Ren Qing;Zeng Siheng(Department of Obstetrics and Gynecology,Hainan Western Central Hospital,Danzhou 571700,China)
出处
《实用妇科内分泌电子杂志》
2024年第21期49-52,共4页
Electronic Journal of Practical Gynecological Endocrinology
基金
海南省卫生健康行业科研项目(编号22A200159)。
