摘要
以中国红豆杉为试验材料,基于其全长转录组测序数据,采用生物信息学手段筛选和鉴定TcDof基因家族的方法,对其理化性质、保守结构域、系统进化树及中国红豆杉剥皮再生过程中基因表达模式进行分析,以期为中国红豆杉TcDofs基因的功能研究提供参考依据。结果表明:中国红豆杉共含有39个具有完整ORF的Dof基因,长度为534~1599 bp,氨基酸数目为177~532 aa,等电点介于5.45~9.81,所有TcDofs基因定位在细胞核中,TcDofs具有典型的C2-C2单锌指结构。系统进化树分析发现,TcDof蛋白分为A、C、D 3个亚家族,含有8个保守基序,所有的Dof蛋白均具有Motif 1保守基序。TcDof1、TcDof26、TcDof38基因在中国红豆杉剥皮再生过程中的表达水平呈现明显上调,推测其调控中国红豆杉的剥皮组织再生过程。
Taking Taxus chinensis as the experiment material,based on the full-length transcriptome data of T.chinensis,with the application of bioinformatics methods,TcDof family genes were screened and identified,the physical and chemical properties,conservative domain structure,phylogenetic tree,and the expression profiles of the TcDof genes in tissue regeneration after bark girdling were analyzed,in order to provide reference for further analysis of the biological function of TcDofs genes in T.chinensis.The results showed that the total of 39 Dofs contained open reading frame(ORF).The coding region of TcDofs were 534-1599 bp in length,their numbers of amino acid were between 177 aa and 532 aa,their isoelectric point were between 5.45 and 9.81,all TcDofs location were in the cell nucleus.The TcDofs had a typical C2-C2 single zinc finger structure.Phylogenetic tree analysis found that Taxus chinensis could be divided into A,C,D three subfamily containing 8 conserved motifs,and all TcDofs proteins had the Motif 1 conserved motif.The expression levels of TcDof1,TcDof38,and TcDof26 genes were significantly upregulated during the bark regeneration process of Taxus chinensis,suggesting that they regulate the bark tissue regeneration of Taxus chinensis.
作者
李艳艳
尚欣悦
吴停勋
王俊青
LI Yanyan;SHANG Xinyue;WU Tingxun;WANG Junqing(Pingdingshan University,Pingdingshan,Henan 467000;Henan Provincial Key Laboratory of Germplasm Innovation and Utilization of Eco-Economic Woody Plants,Pingdingshan,Henan 467000)
出处
《北方园艺》
北大核心
2025年第4期15-23,共9页
Northern Horticulture
基金
国家自然科学基金资助项目(31700597)
河南省科技攻关资助项目(222102310237)
河南省高等学校重点科研资助项目(22B350005)
河南省卫生健康委员会研究资助项目(WJLX2023158)
平顶山学院培育基金资助项目(2022-JY37)。
关键词
中国红豆杉
Dof家族
进化树
表达分析
Taxus chinensis
Dof family
phylogenetic tree
expression analysis
