摘要
鉴于当前糖类植物免疫诱抗剂产品质量分析检测方法研究相对匮乏,本研究系统评估了硫酸-苯酚法、硫酸-蒽酮法及Elson-Morgan法在多种糖类化合物检测中的适用性及影响因素,并建立了有效的比色分析方法。结果表明,三种比色法对葡萄糖、半乳糖醛酸和氨基葡萄糖呈现不同的响应性,单糖组成对比色法的选择尤为重要。以相应单糖组成的葡聚糖、多聚半乳糖醛酸和氨基寡糖素为代表聚糖化合物,通过优化比色反应条件,确立了适合的最佳检测条件。方法验证结果显示,各方法在其线性范围内均表现出优异的线性相关性(R^(2)>0.998)、良好的重复性(相对标准偏差范围为0.4%~0.9%)及高回收率(100%~101%)。结合离子色谱法揭示了当前低聚糖素制剂的有效成分为低聚氨基葡萄糖,并指出了当前登记资料中对于糖类有效成分界定不清晰的现状。最后,本研究确立并验证了用于香菇多糖、低聚糖素和几丁聚糖制剂的比色分析方法,结果均展现出了良好的线性关系、优异的重现性和高准确度,满足产品质量检测的方法要求。此研究为糖类植物免疫诱抗剂质量分析提供了策略与方法支持,有助于推动产业健康发展。
Given the limited research on current methods for detecting the product quality of sugarbased plant immunity inducers,this study systematically evaluated the applicability and influencing factors of the sulfuric acid-phenol method,sulfuric acid-anthracenone method,and Elson-Morgan method for detecting various sugar active ingredients in sugar plant immunity inducer formulations,and effective colorimetric methods were established.Results showed that the three colorimetric methods presented different responsiveness to glucose,galacturonic acid and glucosamine,and the composition of monosaccharides was particularly important for the selection of colorimetric methods.Dextran,polygalacturonic acid and oligosaccharins,which composed of the corresponding monosaccharides,were used as representative glycan compounds,and suitable methods for these polysaccharide compounds were established by optimizing the colorimetric conditions.Method validation results showed excellent linear correlation(R^(2)>0.998),good reproducibility(relative standard deviation range of 0.4%-0.9%)and high recoveries(100%-101%).Combined with ion chromatography,this study revealed that the active ingredients of current oligosaccharin formulations are oligo-glucosamine,and pointed out that the active ingredients of the sugar plant immunity inducers were not clearly defined in the current registration data.Furthermore,the present study established and validated the colorimetric methods for analyzing the fungous proteoglycan,oligosaccharins and chitosan formulations,and the methods presented good linearity,excellent reproducibility and high accuracy,which met the requirements for the product quality analysis methods of formulations.This study provides strategies and method support for the quality analysis of sugar plant immunity inducers and helps to promote the healthy development of related industries.
作者
高志强
刘霁萱
刘丰茂
赵鹏跃
郭丹
王文卓
GAO Zhiqiang;LIU Jixuan;LIU Fengmao;ZHAO Pengyue;GUO Dan;WANG Wenzhuo(Innovation Center for Pesticide,Department of Applied Chemistry,College of Science,China Agricultural University,Beijing 100193,China;Institute of Plant Protection,Chinese Academy of Agricultural Sciences,Beijing 100193,China;Shenyang SYRICI Testing Co.,Ltd.,Shenyang 100021,China;Institute for the Control of Agrochemicalsy,Beijing 100125,China)
出处
《农药学学报》
北大核心
2025年第1期126-137,共12页
Chinese Journal of Pesticide Science
基金
农业标准制修订项目(No.NYB-22335).
