摘要
背景:人多能干细胞来源心肌细胞为研究心脏疾病、药物筛选、体外心脏建模及潜在的细胞治疗方法等提供了理想的细胞来源。然而,人多能干细胞来源心肌细胞属于未成熟细胞,主要表现为缺乏特定基因表达,Ca^(2+)处理水平低及结构、代谢、电生理特性等不成熟,严重阻碍了人多能干细胞来源心肌细胞的应用。目的:综述通过体外合成微环境促进人多能干细胞来源心肌细胞成熟的学术进展及临床应用。方法:检索中国知网、万方数据库、维普数据库、PubMed、Web of Science和Medline数据库,以“human pluripotent stem cells,human myocardial cells,hPSC-CMs,mature,OA,human pluripotent stem cell-derived cardiomyocytes,hPSC-CMs”为英文检索词,以“人多能干细胞,心肌细胞,成熟,OA,hPSC-CMs”为中文检索词,检索2002年1月至2024年7月发表的所有相关文献,最后纳入82篇文献进行综述。结果与结论:(1)近年来,体外合成微环境由于刚度、可塑性、纳米级形貌和化学功能等优良固有特性而受到广泛关注;(2)人多能干细胞来源心肌细胞可作为治疗心血管疾病的有效平台;(3)机械刺激、电刺激、生物化学分子添加及三维培养法等作为促人多能干细胞来源心肌细胞成熟的有效方法,可进一步推进人多能干细胞来源心肌细胞在临床的应用。
BACKGROUND:Human pluripotent stem cell-derived cardiomyocytes offer an ideal cellular resource for studying heart diseases,conducting drug screening,developing in vitro heart models,and exploring potential cell therapies.However,human pluripotent stem cell-derived cardiomyocytes are characterized by immaturity with limited specific gene expression,low Ca^(2+) processing levels,and underdeveloped structural,metabolic,and electrophysiological features.These limitations significantly impede the application of human pluripotent stem cell-derived cardiomyocytes.OBJECTIVE:To review the academic progress and clinical application of promoting the maturation of human pluripotent stem cell-derived cardiomyocytes by in vitro synthetic microenvironment.METHODS:CNKI,WanFang,VIP,PubMed,Web of Science,and Medline databases were searched,with“human pluripotent stem cells,human myocardial cells,hPSC-CMs,mature,OA,human pluripotent stem cell-derived cardiomyocytes,hPSC-CMs”as English search terms and“human pluripotent stem cells,cardiomyocytes,mature,OA,hPSC-CMs”as Chinese search terms.All relevant literature published from January 2002 to July 2024 was retrieved and 82 articles were included in the review.RESULTS AND CONCLUSION:(1)In recent years,in vitro synthetic microenvironments have attracted extensive attention due to their excellent intrinsic properties such as stiffness,plasticity,nanoscale morphology,and chemical functionality.(2)Human pluripotent stem cell-derived cardiomyocytes can be used as an effective platform for the treatment of cardiovascular diseases.(3)Mechanical stimulation,electrical stimulation,addition of biochemical molecules,and three-dimensional culture methods are effective methods to promote the maturation of human pluripotent stem cell-derived cardiomyocytes,which can further promote the clinical application of human pluripotent stem cell-derived cardiomyocytes.
作者
刘璐
钟畅
余欣
任晨媛
巩杨杨
周平
王迎斌
Liu Lu;Zhong Chang;Yu Xin;Ren Chenyuan;Gong Yangyang;Zhou Ping;Wang Yingbin(Second Clinical Medical College of Lanzhou University,Lanzhou 730000,Gansu Province,China;School of Stomatology,Lanzhou University,Lanzhou 730000,Gansu)
出处
《中国组织工程研究》
北大核心
2025年第36期7856-7862,共7页
Chinese Journal of Tissue Engineering Research
基金
甘肃省自然科学基金项目(24JRRA1106),项目负责人:王迎斌。
关键词
体外合成微环境
人多能干细胞
心肌细胞
细胞成熟
物理方法
生物化学方法
三维培养法
临床应用
综述
in vitro synthetic microenvironment
human pluripotent stem cell
cardiomyocyte
cell maturation
physical method
biochemical method
three-dimensional culture method
clinical application
review
