摘要
Saline-alkaline soils are a major environmental problem that limit plant growth and crop productivity.Plasma membrane H^(+)-ATPases and the salt overly sensitive(SOS)signaling pathway play important roles in plant responses to saline-alkali stress.However,little is known about the functional genes and mechanisms regulating the transcription of H^(+)-ATPases and SOS pathway genes under saline–alkali stress.In the present study,we identified that the plant AT-rich sequence and zinc-binding(TaPLATZ2)transcription factor are involved in wheat response to saline-alkali stress by directly suppressing the expression of TaHA2/TaSOS3.The knockdown of TaPLATZ2 enhances salt and alkali stress tolerance,while overexpression of TaPLATZ2 leads to salt and alkali stress sensitivity in wheat.In addition,TaWRKY55 directly upregulated the expression of TaPLATZ2 during saline-alkali stress.Through knockdown and overexpression of Ta WRKY55 in wheat,Ta WRKY55 was shown to negatively modulate salt and alkali stress tolerance.Genetic analyses confirmed that Ta PLATZ2 functions downstream of Ta WRKY55 in response to salt and alkaline stresses.These findings provide a TaWRKY55–TaPLATZ2–TaHA2/TaSOS3 regulatory module that regulates wheat responses to saline-alkali stress.
基金
supported by grants from the Agricultural Variety Improvement Project of Shandong Province(2022LZGC002)
the National Natural Science Foundation of China(32171935 and 32372039)
the National Key Research and Development Program of China(2022YFD1201700)
the Natural Science Foundation of Shandong Province(ZR2019ZD16)
the Intramural Joint Program Fund of State Key Laboratory of Microbial Technology(SKLMTIJP2024-06)。
