摘要
目的:建立槐花、炒槐花标准汤剂特征图谱,区分图谱差异。方法:采用了UPLC色谱仪与WATERS BEH C18(2.1mm×100 mm,1.7μm)色谱柱,以乙腈-0.1%磷酸水为流动相,柱温为35℃,流速为0.3 mL/min,检测波长为257 nm。结果:应用特征图谱提取槐花、炒槐花标准汤剂特征图谱中特征成分,共确定5个特征峰。结论:槐花、炒槐花标准汤剂UPLC特征图谱方法可靠、稳定,可用于槐花、炒槐花配方颗粒的定性鉴别。
Objective:To establish the feature chromatograms of standard decoction of Huaihua(Sophora japonica L)and fried one,and to distinguish the differences in the mapping.Methods:A UPLC chromatographic instru-ment and WATERS BEH C18(2.1 mm×100 mm,1.7μm)chromatographic column were employed,with acetonitrile-0.1%aqueous phosphate as the mobile phase,column temperature 35℃,flow rate 0.3 mL/min and the detection wavelength 257 nm.Results:Five characteristic peaks were confirmed through the extraction of characteristic components from standard decoction of Huaihua and fried Huaihua with characteristic mapping.Conclusion:The method of UPLC characteristic spectrum of Huaihua and fried Huaihua,which is reliable and stable,could be used for qualitative identification of the formula granules.
作者
高晗
孙福仁
李争
李军山
梁彩娟
廖明丽
李振江
GAO Han;SUN Furen;LI Zheng;LI Junshan;LIANG Caijuan;LIAO Mingli;LI Zhenjiang(China Shineway Pharmaceutical Group Ltd.,Shijiazhuang 051430,China;Shijiazhuang Industrial Technology Research Institute of Traditional Chinese Medicine Formula Granules,Shijiazhuang 050002,China;Yunnan Shineway Spirin Pharmaceutical Co.,Chuxiong 675000,China)
出处
《西部中医药》
2025年第1期11-17,共7页
Western Journal of Traditional Chinese Medicine
基金
云南省重大科技专项计划(202102AA310027)
云南省配方颗粒重点实验室项目(202105AG070014)
石家庄市中药配方颗粒产业技术研究院项目(228790779A)。
关键词
槐花
炒槐花
标准汤剂
特征图谱
芦丁
槲皮素
FISHER判别分析
Huaihua
fried Huaihua
standard decoction
characteristic mapping
rutin
quercetin
Fisher discriminant analysis
