摘要
目的 探究YTH N6甲基腺苷RNA结合蛋白3(YTHDF3)对人结直肠癌细胞系HCT-116增殖、迁移和凋亡的影响。方法 将细胞分为阴性对照组、干扰组1、干扰组2、干扰组3,在结直肠癌细胞HCT-116中转染小干扰YTHDF3,通过qRT-PCR和Western blot实验检测各组细胞YTHDF3表达。采用CCK-8实验、平板克隆实验检测各组细胞增殖能力,Transwell实验和划痕实验检测各组细胞迁移能力,流式细胞术检测各组细胞凋亡能力。结果 通过qRT-PCR和Western blot实验验证HCT-116细胞沉默效率。与阴性对照组相比,在HCT-116细胞中转染小干扰YTHDF3后,干扰组2和干扰组3能有效降低YTHDF3的表达;沉默YTHDF3能显著抑制HCT-116细胞增殖和迁移,促进细胞凋亡。结论 沉默YTHDF3能够减缓HCT-116细胞增殖和迁移,促进HCT-116细胞凋亡。
Objective To investigate the impact of YTH N6 methyladenosine RNA binding protein 3 (YTHDF3) on the proliferation,migration,and apoptosis of human colorectal cancer cell line HCT-116.Methods The cells were divided into a negative control group,interference groups 1,2,and 3.Small interfering RNA targeting YTHDF3 was transfected into colorectal cancer cells HCT-116.The expression of YTHDF3 in each group was assessed using qRT-PCR and Western blot analysis.Cell proliferation was evaluated using CCK-8 assay and plate cloning assay,while cell migration was assessed using transwell assay and scratch assay.Flow cytometry was used to measure cell apoptosis.Results The silencing efficiency of HCT-116 cells was validated through qRT-PCR and Western blot analysis.Transfection of small interfering YTHDF3 effectively reduced the expression of YTHDF3 in HCT-116 cells compared to the negative control group.Silencing YTHDF3 can significantly inhibited cell proliferation and migration while promoting apoptosis in HCT-116 cells.Conclusion Silencing YTHDF3 can decelerates cell proliferation and migration while enhancing apoptosis in HCT-116 cells.
作者
周涛
付溶川
唐熙峻
孙雨
郑帅
赵恩宏
肖丽君
ZHOU Tao;FU Rong-chuan;TANG Xi-jun;SUN Yu;ZHENG Shuai;ZHAO En-hong;XIAO Li-jun(Basic Medical Institute of Chengde Medical University,Chengde,Hebei,067000,China;Affiliated Hospital of Chengde Medical University,Chengde,Hebei,067000,China)
出处
《承德医学院学报》
2025年第1期1-6,共6页
Journal of Chengde Medical University
