摘要
为探究活性污泥高负荷运行时发生污泥膨胀的原因及控制策略,考察了污泥负荷对污泥性质、微生物群落整体结构、丝状菌属、胞外聚合物分泌菌属、参与胞外多糖合成功能基因簇等的影响,并研究了投加香兰素对污泥膨胀的控制效果.结果表明,污泥发生膨胀后,胞外聚合物中蛋白质与多糖之比下降,限制了蛋白质发挥生物絮凝作用,导致污泥沉降性能变差.高负荷运行时发生污泥膨胀的成因是Sphaerotilus主导的丝状菌膨胀和Flavobacterium主导的粘性膨胀.投加香兰素有效抑制了Sphaerotilus繁殖,但仍发生由Thauera和Zoogloea主导的粘性膨胀.宏基因分析揭示,参与胞外多糖合成的alg、eps和pel功能基因簇丰度在膨胀污泥中分别增加了0.4~0.5倍、0.8~1.1倍和10.3~15.7倍,是胞外多糖含量上升的潜在成因.
To investigate the causes of sludge bulking during high load operation of activated sludge and the control effect of adding vanillin,this study examined the overall changes in microbial community,filamentous bacteria genus,extracellular polymeric substance secreting bacteria genus,and functional gene clusters involved in exopolysaccharides synthesis when operating under conventional load or high load with or without vanillin addition.The results indicated that after sludge bulking occurred,there was a decrease in the proportion of proteins to polysaccharides in extracellular polymeric substances.This reduction limited the ability of proteins to act as a biological flocculant and resulted in poorer settling performance of the sludge.The causes of sludge bulking during high load operation were attributed to filamentous bacteria dominated by Sphaerotilus and viscous bulking led by Flavobacterium.The addition of vanillin effectively inhibited the proliferation of Sphaerotilus but still allowed for viscous bulking dominated by Thauera and Zoogloea.Metagenomic sequencing analysis revealed that the abundance of alg,eps,and pel functional gene clusters involved in exopolysaccharide synthesis increased by 0.4~0.5 times,0.8~1.1 times,and 10.3~15.7 times,respectively.These findings suggest that these gene clusters may be potential factors contributing to the substantial increase in exopolysaccharide content observed in bulking sludge.
作者
安莹
张慧敏
刘韵
陈广
唐晨
周振
AN Ying;ZHANG Hui-min;LIU Yun;CHEN Guang;TANG Chen;ZHOU Zhen(College of Environmental and Chemical Engineering,Shanghai University of Electric Power,Shanghai 200090,China;Shanghai Chengtou Waste Water Treatment Co.,Ltd.,Shanghai 201203,China)
出处
《中国环境科学》
北大核心
2025年第2期768-775,共8页
China Environmental Science
基金
国家自然科学基金资助项目(51878403)
上海市科委项目(22dz1209206)。
关键词
污泥膨胀
高负荷运行
香兰素
微生物群落
胞外多糖
sludge bulking
high load operation
vanillin
microbial community
exopolysaccharides
