摘要
Background Enterotoxigenic Escherichia coli(E.coli)is a threat to humans and animals that causes intestinal dis-orders.Antimicrobial resistance has urged alternatives,including Lactobacillus postbiotics,to mitigate the effects of enterotoxigenic E.coli.Methods Forty-eight newly weaned pigs were allotted to NC:no challenge/no supplement;PC:F18^(+)E.coli chal-lenge/no supplement;ATB:F18^(+)E.coli challenge/bacitracin;and LPB:F18^(+)E.coli challenge/postbiotics and fed diets for 28 d.On d 7,pigs were orally inoculated withF18^(+)E.coli.At d 28,the mucosa-associated microbiota,immune and oxidative stress status,intestinal morphology,the gene expression of pattern recognition receptors(PRR),and intestinal barrier function were measured.Data were analyzed using the MIXED procedure in SAS 9.4.Results PC increased(P<0.05)Helicobacter mastomyrinus whereas reduced(P<0.05)Prevotella copri and P.ster-corea compared to NC.The LPB increased(P<0.05)P.stercorea and Dialister succinatiphilus compared with PC.The ATB increased(P<0.05)Propionibacterium acnes,Corynebacterium glutamicum,and Sphingomonas pseudosanguinis compared to PC.The PC tended to reduce(P=0.054)PGLYRP4 and increased(P<0.05)TLR4,CD14,MDA,and crypt cell proliferation compared with NC.The ATB reduced(P<0.05)NOD1 compared with PC.The LPB increased(P<0.05)PGLYRP4,and interferon-γand reduced(P<0.05)NOD1 compared with PC.The ATB and LPB reduced(P<0.05)TNF-αand MDA compared with PC.Conclusions TheF18^(+)E.coli challenge compromised intestinal health.Bacitracin increased beneficial bacteria show-ing a trend towards increasing the intestinal barrier function,possibly by reducing the expression of PRR genes.Lac-tobacillus postbiotics enhanced the immunocompetence of nursery pigs by increasing the expression of interferon-γand PGLYRP4,and by reducing TLR4,NOD1,and CD14.
基金
USDA-NIFA Hatch Fund(#02636,Washington DC,USA),North Carolina Agricultural Foundation(#660101,Raleigh,NC,USA),and Adare Biome(Houdan,France).
