摘要
外侧器官边界域(lateral organ boundaries domain,LBD)转录因子广泛存在于高等植物中,并在植物生长发育和逆境胁迫等过程中发挥了非常重要的作用。沙棘(Hippophae rhamnoides L.)作为一种抗旱、耐寒、耐盐碱的灌木,具有一定的生态价值和经济价值。为了分析LBD基因家族在中国沙棘雌雄花芽发育过程中的作用,本研究利用生物信息学方法从沙棘全基因组数据中鉴定出了11个LBD基因。LBD基因家族不均匀分散在5条染色体上,编码159−302个氨基酸,分子质量为18249.91−33202.01 Da。亚细胞定位预测表明蛋白质分布于细胞核或叶绿体中;LBD序列较为保守,具有高度相似的基序、基因结构和蛋白质三维结构;将沙棘与拟南芥(Arabidopsis thaliana)、大麦(Hordeum vulgare)的LBD基因进行系统发育分析发现,HrLBD基因家族均可细分为ClassⅠ和ClassⅡ两大亚家族;转录组及反转录实时荧光定量聚合酶链式反应(reverse transcriptionpolymerase chain reaction,RT-qPCR)结果显示,HrLBD基因在沙棘雄性花芽发育过程中表达量更高,并随着花芽发育过程表达量逐渐升高,推测HrLBD基因在特定时期影响沙棘雄性花芽发育。本研究为阐明HrLBD基因在沙棘雌雄花芽生长发育中的作用和性别分化机制奠定了理论基础。
Lateral organ boundaries(LOB)domain(LBD)genes encode a family of transcription factors ubiquitous in higher plants,playing crucial roles in the growth,development,and stress responses.Hippophae rhamnoides,known for its drought,cold,and saline-alkali tolerance,offers significant economic benefits and ecological values.Utilizing the whole genome data and bioinformatics approaches,this study identified and analyzed the LBD gene family in H.rhamnoides.Additionally,we examined the expression pattern of HrLBD genes by integrating the transcriptome data from male and female flower buds in development.Eleven LBD genes were identified in H.rhamnoides,and these genes were distributed on five chromosomes.The HrLBD proteins showed the lengths ranging from 159 aa to 302 aa,the molecular weights between 18249.91 Da and 33202.01 Da,and the subcellular localization in the nucleus or chloroplasts.LBD protein domains and gene structures were highly conserved,featuring similar motifs.The phylogenetic analysis of HrLBD genes and the LBD genes in Arabidopsis thaliana and Hordeum vulgare revealed that HrLBD genes falled into two major categories:Class I and Class II.The transcriptome data and RT-qPCR showed that HrLBD genes were highly expressed in male flower buds,with up-regulated expression levels throughout bud development,indicating a role in the specific stage of male flower bud development.This study lays a theoretical foundation for exploring the roles of HrLBD genes in the growth,development,and sex differentiation of H.rhamnoides flower buds.
作者
李昕娟
杨盼盼
张恬
任乾丹
周武
LI Xinjuan;YANG Panpan;ZHANG Tian;REN Qiandan;ZHOU Wu(College of Eco-environmental Engineering,Qinghai University,Xining 810016,Qinghai,China;Xinjiang Key Laboratory of Biological Resources and Genetic Engineering,College of Life Science and Technology,Xinjiang University,Urumqi 830046,Xinjiang,China)
出处
《生物工程学报》
北大核心
2025年第2期753-770,共18页
Chinese Journal of Biotechnology
基金
国家自然科学基金(32160386)
青海省科技厅应用基础研究项目(2024-ZJ-782)。
关键词
沙棘
外侧器官边界域(LBD)基因家族
花芽发育
基因表达
反转录实时荧光定量聚合酶链式反应
Hippophae rhamnoides
lateral organ boundaries(LOB)domain(LBD)gene family
flower bud development
gene expression
reverse transcription-polymerase chain reaction
