摘要
目的调查戊型肝炎病毒(HEV)在献血人群乙型肝炎病毒(HBV)感染者中的血清学感染情况,为HEV的监测、预防及治疗提供科学数据支撑。方法随机收集2022年1—12月武汉血液中心无偿献血者经血液筛查乙肝表面抗原(HBsAg)反应性标本219份和隐匿性乙肝感染(OBI)标本142份(HBV感染人群),以筛查结果正常的标本873份作为对照(正常人群)。采用磁微粒化学发光法测定HBV感染人群乙肝两对半,实时荧光定量PCR测定DNA载量。采用ELISA方法检测标本中的抗-HEV IgG、抗-HEV IgM和HEV Ag。采用χ^(2)检验或Fisher精确检验来评估不同献血人群和不同变量抗-HEV IgG和抗-HEV IgM反应性率的差异。采用多因素Logistic回归分析抗-HEV反应性的潜在危险因素。结果HBV感染人群HBsAg反应性者抗原呈现低水平表达,OBI感染者HBV DNA载量为1~131.43 IU/mL(中位数11.24 IU/mL)。HBV感染人群抗-HEV IgG和抗-HEV IgM的反应性率分别为34.63%和1.11%,其中HBsAg反应性人群抗-HEV IgG和抗-HEV IgM的反应性率分别为30.14%和0,OBI人群抗-HEV IgG和抗-HEV IgM的反应性率分别为41.55%和2.82%;正常人群抗-HEV IgG和抗-HEV IgM的反应性率分别为18.67%和1.49%。经统计学分析,HBV感染人群与正常人群抗-HEV IgG反应性率有差异(34.63%vs 18.67%,P<0.05),抗-HEV IgM反应性率无差异(1.11%vs 1.49%,P>0.05)。2类献血人群均未检出HEV Ag。多因素Logistic回归分析表明,年龄是2类献血人群抗-HEV IgG反应性的独立风险因素。结论武汉地区献血人群HBV感染者抗-HEV IgG反应性率显著高于正常人群,年龄是其独立风险因素。因此,针对HBV感染者,应加强和重视HEV的防治,旨在减少HEV传播和疾病负担。
Objective To investigate the seroprevalence characteristics of hepatitis E virus(HEV)among blood donors with hepatitis B virus(HBV)infection,so as to provide data support for the monitoring,prevention and treatment of HEV.Methods From January to December 2022,219 samples positive for hepatitis B surface antigen(HBsAg),142 occult hepatitis B virus infection(OBI)samples(HBV group)and 873 samples tested negative(control group)were collected.361 samples were further tested with viral load assay and serological testing for five serological markers(HBsAg,HBsAb,HBeAg,HBeAb and HBcAb),and the DNA load was measured using real time fluorescence quantitative PCR.Commercially available enzyme-linked immunosorbent assays(ELISA)were used for the detection of anti-HEV IgG,anti-HEV IgM and HEV antigen(Ag).The Chi-square test or Fisher′s exact test was used to assess the differences in the reactivity rates of anti-HEV IgG and anti-HEV IgM among different blood donor populations and different variables.Multivariable logistic regression was used to examine potential risk factors associated with anti-HEV IgG seroprevalence.Results In the HBV group,HBsAg positive donors exhibited low expression of antigen.The HBV DNA load of OBI infected donors ranged from 1 to 131.43 IU/mL(median 11.24 IU/mL).The prevalence of anti-HEV IgG and IgM antibody in the HBV group were 34.63%and 1.11%,respectively.Among them,the prevalence of anti-HEV IgG and anti-HEV IgM in the HBV group was 34.63%and 0,respectively(P<0.05),while in the OBI donors,they were 41.55%and 2.82%,respectively.In the normal donors,the reactivity rates for anti-HEV IgG and anti-HEV IgM were 18.67%and 1.49%,respectively.Statistical a-nalysis showed that there was a difference in the reactivity rate of anti-HEV IgG between the HBV-infected donors and the normal donors(34.63%vs 18.67%,P<0.05),but no difference in the reactivity rate of anti-HEV IgM(1.11%vs 1.49%,P>0.05).No HEV Ag was detected in either group of blood donors.Multivariate logistic regression analysis indicated that age was an independent risk factor for anti-HEV IgG reactivity in both groups of blood donors.Conclusion The reactivity rate of anti-HEV IgG among HBV-infected blood donors was significantly higher than that in the normal donors in Wuhan,with age being an independent risk factor.Therefore,for HBV-infected donors,it is essential to strengthen and prioritize the prevention and treatment of HEV to reduce the spread of HEV.
作者
余琴
许婷婷
杨浩
赵磊
YU Qin;XU Tingting;YANG Hao;ZHAO Lei(Wuhan Blood Center,Wuhan 430030,China)
出处
《中国输血杂志》
2025年第1期1-6,18,共7页
Chinese Journal of Blood Transfusion
基金
湖北省卫生健康委科研课题项目(WJ2021M023)。
