摘要
为探究旱地红壤微生物群落与不同施肥制度之间的关系,采用宏基因组测序,比较分析长期定位试验地4种施肥处理:不施肥(CK)、施氮磷钾肥(NPK)、施有机肥(OM)、氮磷钾肥和有机肥混合施用(NPKOM)的土壤微生物群落组成及多样性。结果显示:相比对照,施用有机肥和氮磷钾肥和有机肥混合施用显著增加放线菌门(Actinobacteria)和变形菌门(Proteobacteria)的丰度,降低了绿弯菌门(Chloroflexi)的丰度;施肥显著改变微生物群落结构,施用无机肥降低土壤微生物数量,施用有机肥和无机有机混合施用则增加土壤微生物数量;土壤微生物群落在门水平与pH、全磷(TP)、土壤有机质(SOM)、碱解氮(AN)和有效磷(AP)含量显著相关,pH是影响土壤微生物群落结构的关键因素;不同施肥处理下微生物代谢功能差异显著。
Sequencing of the metagenome was used to comparatively analyze the composition and the diversity of microbial communities in soil under the four fertilizations including no fertilization(CK),nitro-gen,phosphorus and potassium fertilizers(NPK),organic fertilizer(OM),and organic fertilizer mixed with nitrogen,phosphorus and potassium fertilizers(NPKOM)in experiments of long-term positioning to study the relationship between the microbial communities in dryland red soils and the systems of fertiliza-tion.The results showed that OM and NPKOM significantly increased the abundance of Actinobacteria and Proteobacteria compared to CK,but decreased the abundance of Chloroflexi.Fertilization significantly changed the structure of microbial community in soil,with OM reducing and NPKOM increasing the num-ber of microorganisms in soil.The microbial community in soil was significantly correlated with pH and the content of total phosphorus(TP),soil organic matter(SOM),alkali-hydrolyzable nitrogen(AN),and available phosphorus(AP)at the level of phylum.pH was a key factor affecting the structure of microbial communities in soil.There were significant differences in metabolic functions of microorganisms under dif-ferent fertilizations.It will provide a certain theoretical basis for the rational fertilization in dryland red soils in China.
作者
余腾
李顺江
万子维
阙正扬
黄国勤
徐慧芳
YU Teng;LI Shunjiang;WAN Ziwei;QUE Zhengyang;HUANG Guoqin;XU Huifang(Research Center for Ecological Sciences,Jiangxi Agricultural University,Nanchang 330045,China)
出处
《华中农业大学学报》
北大核心
2025年第1期148-155,共8页
Journal of Huazhong Agricultural University
基金
国家自然科学基金项目(32160310)
江西省自然科学基金项目(20232BAB215015)。
关键词
长期定位试验
宏基因组学
施肥制度
有机肥
旱地
红壤
土壤微生物群落
experiments of long-term positioning
metagenomics
system of fertilization
organic fer-tilizer
dry land
red soils
microbial community in soil
