摘要
目的 探究等离子体活化气(PAG)促进伤口愈合的效应。方法 通过检测PAG促进细胞增殖和染菌伤口愈合的效果,探究PAG的促愈合效应。细胞增殖实验:将处于对数生长期的5种不同细胞[包括人皮肤成纤维细胞(HSF)、人骨骼肌永生化细胞(IHSMC)、人正常前列腺基质永生化细胞(WPMY-1)、人类永生化表皮细胞(HaCaT)、人包皮成纤维细胞(HFF)]接种于培养板中。待细胞贴壁生长后,用合适的时间梯度对不同细胞各自进行实验分组。在细胞接受不同时间PAG处理后,采用CCK-8方法检测细胞活性。动物染菌伤口愈合实验:用无菌镊子和剪刀在SD大鼠背后创造大小约1.5 cm×1.5 cm的伤口,再用A_(600 nm)=6.5的铜绿假单胞菌(P.Aeruginosa)感染伤口。感染完成后对伤口定期进行PAG处理,根据不同处理时长划分实验组。最终用伤口愈合照片以及伤口相对面积的变化数据作为衡量愈合表现的指标。结果 细胞增殖实验:PAG处理在合理时间内能有效促进细胞增殖,过量则会导致细胞死亡。因此,定义PAG处理细胞的剂量=W/N(J/cell),其中W为活化气装置在处理过程中耗费的功,N为细胞数。使用起效剂量、最佳剂量和安全剂量范围3个指标对PAG的促增殖效应进行表征。动物染菌伤口愈合实验:实验组均表现为伤口愈合速度加快,因此采用最佳处理时间对PAG的促愈合效果进行表征。其中消毒模式起主要作用,最佳处理时间为2 min,促愈模式起辅助作用,整体愈合效果最好的处理条件为消毒模式处理2 min、促愈模式处理1 min。结论 PAG具有促进细胞增殖,促进感染伤口愈合的作用,并且能够总结出指标对其效应进行定量描述,有利于PAG进一步规范化的研究和临床实用。
Objective To explore the effect of plasma-activated gas(PAG)in promoting wound healing.Methods The pro-healing effect of PAG was explored by testing the effectiveness of PAG in promoting cell proliferation and healing of infected wounds.Cell proliferation assay:five different cells(i.e.,HSF,IHSMC,WPMY-1,HaCaT,and HFF)in logarithmic growth phase were inoculated into culture plates.After the cells grew adherently to the wall,the different cells were each grouped experimentally using a suitable time gradient.After the cells were treated with PAG for different time,their activity was detected by CCK-8 method.Animal infected wound healing assay:a wound of about 1.5 cm×1.5 cm in size was created on the back of SD rats with sterile tweezers and scissors,and then the wound was infected with P.Aeruginosa with A_(600 nm)=6.5.After the infection was completed,the wounds were treated with PAG at regular intervals,and the experiment groups were subdivided according to the different treatment durations.Wound healing photographs and changes in relative wound area were used as indicators of healing performance.Results Cell proliferation assay:PAG treatment was effective in promoting cell proliferation for a reasonable period of time,while overdose led to cell death.Therefore,the dose of cells treated with plasma activation gas was defined as=W/N(J/cell),where W is the work consumed by the activation gas device during the treatment process and N is the number of cells.The three indexes(starting dose,optimal dose and safe dose range)were used to characterize the proliferative effect of PAG.Animal infected wound healing assay:the experimental groups all showed accelerated wound healing,so the optimal treatment time was used to characterize the pro-healing effect of PAG.The sterilization mode played a primary role,with an optimal treatment time of 2 min,and the pro-healing mode played a secondary role.The treatment conditions with the best overall healing effect were 2 min for the sterilization mode and 1 min for the pro-healing mode.Conclusion PAG has the effect of promoting cell proliferation and infected wound healing,and indicators can be summarized to quantitatively describe its effect,which is conducive to further standardization of PAG research and clinical utility.
作者
舒江凌
李卓
张紫竹
赵欣怡
张晶硕
祁苗
李想
柳云恩
刘定新
许德晖
SHU Jiangling;LI Zhuo;ZHANG Zizhu;ZHAO Xinyi;ZHANG Jingshuo;QI Miao;LI Xiang;LIU Yun’en;LIU Dingxin;XU Dehui(Centre for Plasma Biomedicine,Xi’an Jiaotong University,Xi’an 710049;School of Life Science and Technology,Xi’an Jiaotong University,Xi’an 710049;Shenyang Medical college,Shenyang 110148;The Second Affiliated Hospital of Xi’an Jiaotong University,Xi’an 710004,China)
出处
《西安交通大学学报(医学版)》
北大核心
2025年第1期43-51,共9页
Journal of Xi’an Jiaotong University(Medical Sciences)
基金
国家自然科学基金资助项目(No.52077166)。
