摘要
为了解蓖麻bZIP基因家族成员的组成和家族进化关系及特征,本研究利用生物信息学鉴定家族成员,进行基因结构、蛋白质保守基序分析及系统发育分析,同时分析其蛋白的理化性质、磷酸化位点和互作关系,以及该基因在组织中的表达模式和表达量。结果表明,共鉴定出46个蓖麻bZIP基因家族成员,位于10个染色体上;基因结构与蛋白质保守基序较为复杂,可分为11个亚族,包含25个基序;与拟南芥进化相似,较为保守;bZIP蛋白为亲水性蛋白,位于细胞核、细胞膜和叶绿体,主要通过丝氨酸的磷酸化来发挥生物学作用,蛋白RcbZIP18、RcbZIP27、RcbZIP2、RcbZIP17和RcbZIP46之间的联系较多,通过蛋白间的相互作用参与蓖麻各个生长时期;该基因家族均参与叶、茎、根和胚等生长时期的发育调控,其中RcbZIP10、RcbZIP25和RcbZIP44在叶片和种子中表现出明显的组织差异性。上述结果为蓖麻bZIP基因家族成员的功能研究提供参考。
To understand the composition,evolutionary relationship and characteristics of Ricinus communis bZIP gene family members,bioinformatics was used to identify family members,analyze gene structure,protein conserved motifs,phylogenetic analysis,and analysis of protein physicochemical properties,phosphorylation sites,and interactions,as well as gene expression patterns and expression levels in tissues.The results showed that 46 members of the Ricinus communis bZIP gene family were identified,which were located on 10 chromosomes.The gene structure and protein conserved motifs were complex,which can be divided into 11 subfamilies,including 25 motifs.Similar to Arabidopsis,the evolution was more conservative;bZIP protein was a hydrophilic protein located in the nucleus,cell membrane and chloroplast.It played a biological role mainly through the phosphorylation of serine.The proteins RcbZIP18,RcbZIP27,RcbZIP2,RcbZIP17 and RcbZIP46 were associated with each other,participating in each growth cycle of Ricinus communis through the interaction between proteins.They are involved in the developmental regulation of leaf,stem,root and embryo during growth,RcbZIP10,RcbZIP25 and RcbZIP44 showed significant organizational differences in leaves and seeds These results provide references for the functional study of Ricinus communis bZIP gene family members.
作者
王冬艳
张春玲
冯研
王丽新
昝睿
狄建军
WANG Dongyan;ZHANG Chunling;FENG Yan;WANG Lixin;ZAN Rui;DI Jianjun(College of Life Science and Food Engineering,Inner Mongolia Minzu University,Tongliao 028000,China;Tongliao Agricultural and Animal Husbandry Academy,Tongliao 028015,China;Inner Mongolia Key Laboratory of Castor Breeding and Comprehensive Utilization,Tongliao 028000,China;Key Laboratory of Castor Breeding of the State Et hnic Affairs Commission,Tongliao 028000,China;Inner Mongolia Industrial Engineering Research Center of Universities for Castor,Tongliao 028000,China;Inner Mongolia Engineering Research Center of Industrial Technology Innovation of Castor,Tongliao 028000,China)
出处
《安徽农学通报》
2025年第2期27-35,共9页
Anhui Agricultural Science Bulletin
基金
内蒙古自治区自然科学基金项目(2024MS03009)
蓖麻产业技术创新内蒙古自治区工程研究中心开放基金项目(MDK2023006)
蓖麻产业技术创新内蒙古自治区工程研究中心开放基金项目(MDK2021002)
内蒙古民族大学博士科研启动基金(BSZ016)
内蒙古自治区蓖麻育种与综合利用重点实验室开放基金项目(MDK2022015)。
关键词
蓖麻
bZIP家族
生物信息学分析
系统进化
表达分析
Ricinus communis L.
bZIP family
bioinformatics analysis
phylogeny
expression analysis
