摘要
基于质量源于设计理念优化藤茶的提取工艺,确定关键的工艺参数及其范围,并采用网络药理学探索关键成分对血脂紊乱的作用机制。选取二氢杨梅素(dyhidromyricetin,DMY)、杨梅苷(myricitrin,MYT)、紫云英苷(astragalin,AST)及杨梅素(myricetin,MYE)作为指标性成分,进行Plackett-Burman实验设计,进行Box-Behnken 2因素4水平实验设计,采取多指标叠加法,用于优化模块Graphical选项进行设计空间构造,并对空间内外的点进行验证。确定最佳提取工艺为提取次数2次,每次提取20min,加溶媒倍数为11.5倍,乙醇体积分数为50%。在此工艺条件下,DMY、MYT、AST、MYE含量均值分别为443.5、125.8、1.9、2.5μg·mg-1,干膏率均值为28.1%。网络药理学研究表明DMY、MYT、AST和MYE共88个关键靶点共同参与脂质代谢紊乱的治疗,蛋白互作网络分析显示TP53、RELA和STAT3等为关键靶点,KEGG富集通路显示关键靶点参与调控脂质与动脉粥样硬化、晚期糖基化终产物及其受体、TNF等信号通路。该提取工艺稳定可行,为后续藤茶相关产品的开发提供一定帮助。
Based on a quality-derived design concept,the extraction process of vine tea was optimized,and the key process parameters and their possible ranges were determined.The functionality of key components in preventing and treating dyslipidemia was explored utilizing network pharmacology.Dihydromyricetin(DMY),myricitrin(MYT),astragalin(AST),and myricetin(MYE)were selected as index components for a Plackett-Burman experimental design,and initial determinations highlighted extraction time and solvent ratio as the key process parameters.On this basis,a Box-Behnken design was employed,incorporating a two-factor and four-level experimental approach.The multi-index superposition method was adopted to optimize the module Graphical option,facilitating the construction of the design space,which was subsequently validated through testing points both inside and outside the designated area.The optimal extraction parameters were determined as two extraction cycles of 20 minutes each,with a solvent-to-sample ratio of 11.5 and a volume fraction of 50%ethanol.The average concentrations of DMY,MYT,AST,and MYE achieved were 443.5,125.8,1.9,and 2.5μg·mg-1,respectively,with a mean dry paste yield of 28.1%.Network pharmacology analysis revealed 88 key targets associated with DMY,MYT,AST,and MYE that contribute collectively to dyslipidemia treatment.The protein-protein interaction analysis identified TP53,RELA,and STAT3 as key targets,and KEGG analysis showed that these key targets are involved in lipid regulation and atherosclerosis pathways,as well as in the signaling pathway of advanced glycation end products(AGEs)and their receptors(RAGE),alongside TNF signaling pathways.The extraction process demonstrated stability and feasibility,thereby providing valuable insights for the future development of products related to vine tea.
作者
赵楠星
袁芳
杨杭艳
夏旭东
叶松霖
陈秋平
ZHAO Nanxing;YUAN Fang;YANG Hanyan;XIA Xudong;YE Songlin;CHEN Qiuping(Zhejiang Wanli University,Ningbo Zhejiang 315100)
出处
《浙江万里学院学报》
2025年第1期80-90,共11页
Journal of Zhejiang Wanli University
基金
浙江省“生物工程”一流学科学生创新项目“对藤茶中降脂活性成分的鉴定及其机理的研究”(CX2021052)。
关键词
藤茶
关键成分
提取工艺优化
设计空间
网络药理学
vine tea
key components
optimization of extraction process
design space
network pharmacology(责任编辑:刘阳雄)
