摘要
目的:探究金丝桃苷对促进烧伤后皮肤成纤维细胞的增殖和胶原合成的作用及其潜在的调控机制。方法:利用高温刺激皮肤成纤维细胞,利用金丝桃苷(0、0.5、1.0、1.5、2.0 mg/ml)处理皮肤成纤维细胞。CCK-8检测细胞增殖能力;qRT-PCR COL1A1和COL3A1的表达;检测Western blot分析CollagenⅠ和CollagenⅢ的表达;免疫荧光检测CollagenⅠ和α-SMA的表达。结果:结果显示金丝桃苷可以促进高温刺激的人真皮成纤维细胞活力。此外,金丝桃苷处理可以提高皮肤成纤维细胞中的胶原合酶表达(COL1A1和COL3A1),以及胶原蛋白CollagenⅠ和CollagenⅢ的表达,进而促进胶原合成。金丝桃苷可以抑制高温刺激的人真皮成纤维细胞中的Wnt3a/β-catenin通路活性。Wnt3a/β-catenin通路抑制剂ICG-001处理可以逆转高温刺激对成纤维细胞活力、胶原合成的抑制作用。结论:金丝桃苷通过抑制Wnt3a/β-catenin通路促进烧伤后皮肤成纤维细胞的增殖和胶原合成。
Objective To explore the effect of hypericin on proliferation and collagen synthesis of skin fibroblasts after burn and its potential regulatory mechanism.Methods Skin fibroblasts were stimulated by high temperature and treated with hypericin(0,0.5,1.0,1.5,2.0 mg/ml).CCK-8 was used to detect cell proliferation.The expression of COL1A1 and COL3A1 by qRT-PCR;The expressions of Collagen I and II were analyzed by Western blot.Collagen I andα-SMA expressions were detected by immunofluorescence.Results In this study,our results show that hypericin can promote the activity of human dermal fibroblasts stimulated by high temperature.In addition,hypericin treatment could enhance the expression of collagen synthase(COL1A1 and COL3A1)and Collagen I and II in skin fibroblasts,thus promoting collagen synthesis.Hypericin inhibits the Wnt3a/β-catenin pathway activity in heat-stimulated human dermal fibroblasts.Treatment with Wnt3a/β-catenin pathway inhibitor ICG-001 reversed the inhibitory effect of high temperature stimulation on fibroblast viability and collagen synthesis.Conclusion In conclusion,hypericin promotes proliferation and collagen synthesis of skin fibroblasts after burn by inhibiting the Wnt3a/β-catenin pathway.
作者
范炜
李烨
FAN Wei;LI Ye(Hospital Office,Baoding Fifth Hospital,Baoding 071000,Hebei,China;Department of Burn and Plastic,Baoding Fifth Hospital,Baoding 071000,Hebei,China)
出处
《中国美容医学》
2025年第1期15-19,共5页
Chinese Journal of Aesthetic Medicine
基金
2023年度河北省中医药管理局科研计划项目指导课题(编号:2023435)。
