摘要
目的探讨脑胶质瘤患者血清miR-186-5p和miR-942-5p表达及临床意义。方法选取2019年10月至2021年12月在该院接受治疗的98例脑胶质瘤患者作为观察组,以及同期在该院体检的101例健康者作为对照组。采用荧光定量PCR(qPCR)法检测血清miR-186-5p和miR-942-5p的表达水平,多因素Cox回归分析脑胶质瘤患者预后的影响因素,受试者工作特征(ROC)曲线及曲线下面积(AUC)分析血清miR-186-5p、miR-942-5p对脑胶质瘤的诊断价值,Kaplan-Meier生存曲线分析血清miR-186-5p、miR-942-5p表达与患者预后的关系。结果观察组血清中miR-186-5p表达水平低于对照组(P<0.05),miR-942-5p表达水平高于对照组(P<0.05);血清miR-186-5p、miR-942-5p单独及联合诊断脑胶质瘤的AUC分别为0.735、0.809、0.895;不同术前Karnofsky功能状态(KPS)评分、世界卫生组织(WHO)分级、局部浸润患者血清miR-186-5p低表达、miR-942-5p高表达比例差异有统计学意义(P<0.05);miR-186-5p高表达患者2年生存率高于miR-186-5p低表达患者(χ^(2)=6.455,P=0.011);miR-942-5p高表达患者2年生存率低于miR-942-5p低表达患者(χ^(2)=9.858,P=0.002);miR-186-5p是脑胶质瘤患者死亡的保护因素(P<0.05),miR-942-5p是危险因素(P<0.05)。结论脑胶质瘤患者血清miR-186-5p表达水平降低、miR-942-5p表达水平升高,二者对脑胶质瘤的发生具有一定的诊断价值。
Objective To investigate the expression levels of serum miR-186-5p and miR-942-5p in patients with glioma and their clinical significance.Methods A total of 98 patients with glioma who were treated in this hospital from October 2019 to December 2021 were selected as the monitored group,and 101 healthy individuals who underwent physical examinations a the same time were selected as the control group.Quantitative fluorescent PCR(qPCR)method was applied to detect the expression levels of miR-186-5p and miR-942-5p in serum,and multivariate COX regression was applied to analyze the prognostic factors of glioma patients.Receiver operating characteristic(ROC)curve and area under curve(AUC)were used to analyze the diagnostic value of serum miR-186-5p and miR-942-5p in glioma.Kaplan-Meier survival curve was used to analyze the relationship between serum miR-186-5p and miR-942-5p expression and prognosis of patients.Results The expression level of serum miR-186-5p in monitored group was lower than that in the control group(P<0.05),and the expression level of miR-942-5p was higher than that in the control group(P<0.05).The AUC of serum miR-186-5p and miR-942-5p in the diagnosis of glioma alone and in combination were 0.735,0.809 and 0.895,respectively.There were significant differences in the proportion of low miR-186-5p expression and high miR-942-5p expression in serum of patients with different preoperative Karnofsky performance status(KPS)scores,World Health Organization(WHO)grades and local infiltration(P<0.05).The 2-year survival rate of patients with high expression of miR-186-5p was higher than that of patients with low expression of miR-186-5p(χ^(2)=6.455,P=0.011).The 2-year survival rate of patients with high miR-942-5p expression was lower than that of patients with low miR-942-5p expression(χ^(2)=9.858,P=0.002).miR-186-5p was a protective factor for mortality in glioma patients(P<0.05),while miR-942-5p was a risk factor(P<0.05).Conclusion Serum miR-186-5p expression level decreases and miR-942-5p expression level increases in glioma patients,both of which have certain diagnostic value for the occurrence of glioma.
作者
李文生
招春飞
姚杰钦
许成杰
肖健齐
蒋海平
张春雨
龙霄翱
LI Wensheng;ZHAO Chunfei;YAO Jieqin;XU Chengjie;XIAO Jianqi;JIANG Haiping;ZHANG Chunyu;LONG Xiaoao(Department of Neurosurgery,Affiliated Hospital of Guangdong Medical University,Zhanjiang,Guangdong 524000,China;Geriatric Ward,Affiliated Hospital of Guangdong Medical University,Zhanjiang,Guangdong 524000,China)
出处
《国际检验医学杂志》
2025年第1期81-85,共5页
International Journal of Laboratory Medicine
基金
湛江市科学技术局资助项目(2020B01446,2020B01389)。
