摘要
【目的】筛选‘羌脆李’果实发育过程中qRT-PCR实验合适的内参基因。【方法】选取7个(18S、Actin、EF1-α、RPL13、RPⅡ、TUB3、EF-2)常用内参基因,对‘羌脆李’果实发育过程中果皮与果肉进行表达稳定性分析,使用geNorm、NormFinder、BestKeeper和RefFinder软件筛选最稳定的内参基因,并用PAL1和PAL3基因验证参考基因表达水平的稳定性。【结果】‘羌脆李’果实发育过程中,TUB3表达最稳定,可用于后续对李果实发育期进行基因表达的内参基因。【结论】运用4种不同的内参基因稳定性分析软件可以筛选出‘羌脆李’果实发育过程中最稳定的内参基因,该结果可为李果实发育过程内参基因的选择提供参考。
【Objective】To screen suitable internal reference genes for qRT-PCR test in the fruit development of'Qiangcuili'.【Method】Seven commonly used internal reference genes(18S,Actin,EF1-α,RPL13,RP-Ⅱ,TUB3,EF-2)were selected to analyze the expression stability of pericarp and flesh during the development of'Qiangcuili'.geNorm,NormFinder,BestKeeper and RefFinder were used to assess the most stable internal reference genes.PAL1 and PAL3 gene were further employed to validate the expression stability of reference genes.【Result】The expression of TUB3 was the most stable during the fruit development of'Qiangcuili',which could be used as an internal reference gene for gene expression in the subsequent fruit development period.【Conclusion】Four different internal reference gene stability analysis softwares can be used to screen out the most stable internal reference genes during the development of plum fruit,and the results can provide reference for the selection of internal reference genes during the development of plum fruit.
作者
唐孝富
孟银银
杜莹
王苗
蒲靖
汪红霞
牛洁
邓群仙
张慧芬
TANG Xiaofu;MENG Yinyin;DU Ying;WANG Miao;PU Jing;WANG Hongxia;NIU Jie;DENG Qunxian;ZHANG Huifen(College of Horticulture,Sichuan Agricultural University,Chengdu 611130,China)
出处
《四川农业大学学报》
CSCD
北大核心
2024年第6期1220-1225,1231,共7页
Journal of Sichuan Agricultural University
基金
四川省自然科学基金(2022NSFSC0092)
四川农业大学学科建设双支计划(2021993327)。
