摘要
目的评价自制的海藻纤维敷料联合表皮生长因子(EGF)对伤口的促愈作用。方法对制成的海藻纤维敷料进行微观形貌结构、透气性、化学结构检测,细胞计数试剂盒8(CCK8)和Calcein-AM/PI检测不同浓度敷料浸提液[0(对照组)、100%(敷料组)]对人胚肺成纤维细胞(HELF)的细胞活性与细胞存活的影响。构建背部全层皮肤缺损创面小鼠模型,并按照不同处理方法分为模型组、纱布组、纱布+EGF组、敷料组、敷料+EGF组,每组12只。观察各组小鼠皮肤损伤后伤口创面愈合情况,计算愈合第3、7、11、15天创面愈合率。愈合第3、7、11天取伤口处及周围皮肤组织进行组织包埋切片,行苏木精-伊红(HE)染色、Masson染色、免疫组化观察创面新生上皮生长情况、炎症细胞浸润程度、胶原纤维沉积情况。结果海藻纤维敷料内部呈三维网状结构,表面平整光滑,经过烘干处理后无残余的纤维丝附着,透气性好。敷料组细胞存活率、细胞活性与对照组比较差异无统计学意义(P>0.05)。愈合第3天,敷料+EGF组创面愈合率在各组中最高(P<0.05);到第15天,除纱布组和模型组外,其余组创面愈合良好。HE染色显示,愈合第7天敷料+EGF组新生上皮长度较其他组更长、炎症细胞数更少(P<0.05);到第11天,敷料组、纱布+EGF组、敷料组+EGF组炎症细胞数比较差异无统计学意义(P>0.05),但均少于纱布组和模型组(P<0.05)。CD31免疫组化和Masson染色显示,愈合第11天敷料+EGF组CD31阳性表达更强,新生血管数量更多、管径更大,胶原纤维最致密。结论自制的海藻纤维敷料多孔、透气、吸湿性和生物相容性好,无细胞毒性,可吸收,联合EGF后有较好的促愈作用,能更好地促进新生上皮增长、减缓炎症细胞浸润、促进新生血管生成和胶原纤维沉积。
Objective To evaluate the effect of self-made seaweed fiber dressing combined with epidermal growth factor(EGF)for promoting the wound healing.Methods The micro-morphology and structure,air permeability and chemical structure of the prepared seaweed fiber dressings were tested.The effects of different concentrations of dressing leach liquor[0(control group),100%(dressing group)]on the cell activity and cellular survival of human embryonic lung fibroblasts(HELF)were detected by the cell counting kit-8(CCK8)and Calcein-AM/PI.The mouse model of full-layer back skin defect wound was constructed and divided into the model group,gauze group,gauze+EGF group,dressing group and dressing+EGF group according to different treatment methods,12 cases in each group.The wound healing of mice after skin injury in each group was observed and the wound healing rate on 3,7,11,15 d was calculated.The tissues of wound surface and surrounding skin were taken on 3,7,11 d of heal for conducting the tissue embedding section,hematoxylin-eosin staining and Masson staining.The immunohistochemistry was used to observe the growth situation of new epithelial,degree of inflammatory cell infiltration and deposition of collagen fibers in the wound surface.Results The inside of the seaweed fiber dressing showed the three-dimensional network structure and smooth surface.After drying treatment,there was no residual fiber attachment with good air permeability.The cell survival rate and cell viability had no statistical difference between the dressing group and control group(P>0.05).On 3 d of heal,the healing rate of wound surface in the dressing+EGR group was the highest among the various groups(P<0.05),on 15 d,except for the gauze group and model group,the heal in the other groups was good.The HE staining showed that on 7 d of healing,the length of the new epithelium in the dressing+EGF group was longer and the inflammatory cells were less than those in the other groups(P<0.05).On 11 d,the number of inflammatory cells had no statistical difference among the dressing group,gauze+EGF group and dressing+EGF group(P>0.05),but all were less than that in the gauze group and model group(P<0.05).CD31 immunohistochemistry and Masson staining showed that the CD31 positive expression on 11 d of heal in the dressing+EGF group was stronger,the new vessels were more,the vessel diameter was greater and collagenous fiber was densest.Conclusion Seaweed fiber dressing is porous,breathable,hygroscopic and biocompatible,non-cytotoxic and absorbable.Combining with EGF has good promotive effect for the growth of new epithelium,could slow down the infiltration of inflammatory cells and promote the formation of new blood vessels and the deposition of collagen fibers.
作者
李程琳
王爽
LI Chenglin;WANG Shuang(School of Basic Medicine,Qingdao University,Qingdao,Shandong 266071,China)
出处
《重庆医学》
CAS
2024年第24期3732-3738,3743,共8页
Chongqing Medical Journal
基金
国家自然科学基金项目(82001754)。
