摘要
目的探讨临床感染性疾病所分离鲍曼不动杆菌(acinetobacter baumarmii,AB)的生物膜基因、外排泵基因及生物膜形成能力与耐药性的相关性。方法收集非重复鲍曼不动杆菌共119株(多重耐药鲍曼不动杆菌MDR-AB菌株74株,非多重耐药菌株45株),PCR检测鲍曼不动杆菌外排泵基因(adeB、adeJ、adeG、adeS和adeR)和生物膜相关基因(bap、ompA、csuA、csuB、csuC、csuD、csuE、abaI、bfmR、bfmS),应用24孔细胞培养板体外构建细菌生物膜,通过结晶紫染色法定性观察所有菌株是否形成生物膜。结果鲍曼不动杆菌对常用抗菌药物均多数为耐药,对替加环素、多粘菌素B、米诺环素较为敏感;生物膜相关基因的总体检出率约80%左右,多重耐药菌中bap、csuB、csuC、csuD基因检出率显著高于非多重耐药菌(P<0.05);主动外排泵基因的检出率不到70%,其中adeB、adeJ、adeS和adeR基因在多重耐药菌中的检出率明显高于非多重耐药菌(P<0.05);而adeG携带率相反。有3株多重耐药菌株及5株非多重耐药菌株未携带有上述外排泵相关基因,所有菌株均未检出abeM基因;95.80%菌株可观察到生物膜的形成。结论医院内检出的鲍曼不动杆菌多呈现出多重耐药特点,多数具有较强的生物膜形成能力,多重耐药菌生物膜基因和外排泵基因携带率更高,可能参与调控形成其多重耐药性。
Objective To investigate the correlation between biofilm gene,efflux pump gene,biofilm forming ability and drug resistance of Acinetobacter baumarmii(AB)isolated from the patients of clinical infectious diseases.Methods A total of 119 non-repeating Acinetobacter baumannii isolates(74 MDR-AB strains and 45 non-multi-drug resistant strains)were collected.The effector pump genes(adeB,adeJ,adeG,adeS and adeR)and biofilm-related genes(bap,ompA,csuA,csuB,csuC,csuD,csuE,abaI,bfmR,bfmS)were detected by PCR.Bacterial biofilm was constructed in vitro with 24-well cell culture plates,and the formation of biofilm was qualitatively observed by crystal violet staining.Results Acinetobacter baumannii was resistant to most of the commonly used antibiotics and sensitive to tigacycline,polymyxin B and minocycline.The overall detection rate of biofilm-related genes was about 80%,and the detection rates of bap,csuB,csuC and csuD genes in multi-drug resistant bacteria were significantly higher than those in non-multi-drug resistant bacteria(P<0.05).The detection rate of active effector pump genes was less than 70%,and the detection rate of adeB,adeJ,adeS and adeR genes in multidrug-resistant bacteria was significantly higher than that in non-multidrug-resistant bacteria(P<0.05).The adeG carrying rate was opposite.Three multidrug-resistant strains and five non-multidrug-resistant strains did not carry the above efflux pump related genes,and no abeM gene was detected in all strains.The formation of biofilm was observed in 95.80%of strains.Conclusion Acinetobacter baumannii in hospital is mostly multidrug-resistant strains,most of which have strong biofilm forming ability.Multidrug-resistant bacteria have higher biofilm gene and effluent-pump gene carrying rate,which may be involved in the regulation and formation of multidrug resistance.
作者
王佳
冯磊
郑玉磊
袁勇
姚瑶
虎子单
申妮
余艳
丁家伟
WANG Jia;FENG Lei;ZHENG Yulei;YUAN Yong;YAO Yao;HU Zidan;SHEN Ni;YU Yan;DING Jiawei(Dept.of Clinical Laboratory,Yan’an Hospital Affiliated to Kunming Medical University,Kunming Yunnan 650051;Dept.of Clinical Laboratory,Panlong District People’s Hospital,Kunming Yunnan 650201,China)
出处
《昆明医科大学学报》
CAS
2024年第12期115-121,共7页
Journal of Kunming Medical University
基金
云南省科技厅-昆明医科大学基础研究联合专项(202201AY070001-187)
云南省科技厅-昆明医科大学基础研究联合专项(202201AY070001-182)
云南省心脏疾病临床医学中心研究项目(ZX2019-08-01)。
关键词
鲍曼不动杆菌
多重耐药
外排泵
生物膜
Acinetobacter baumannii
Multiple drug resistance
Efflux pump
Biofilm
