摘要
金毛鳞伞(Pholiotaaurivella)是野生食药用菌品种之一,在我国广泛分布,但目前对其发掘利用有限。为进一步充分开发和利用该菌种质资源,试验以采自西藏林芝市西藏农牧学院校园内柳树上的一株野生食用菌,编号为HC-1为材料。对该菌子实体形态、菌丝、孢子等进行形态学观察和ITS序列测定,通过系统发育分析比较,将该菌株鉴定为金毛鳞伞(P.aurivella)。通过单因素试验和正交试验对该菌株菌丝的最适生长条件进行生物学特性研究。结果表明:菌株HC-1菌丝生长的最佳碳源为蔗糖,最佳氮源为酵母膏,最适pH为7.0,最适温度为25℃,最佳无机盐为磷酸二氢钾,最适生长因子为维生素C。正交试验表明,菌株HC-1菌丝生长的最佳配方为蔗糖25.0 g·L^(-1),酵母膏3.0 g·L^(-1),pH为7.0,硫酸镁2.0g·L^(-1)。
Pholiota aurivella is one of the wild edible and medicinal fungi,which is widely distributed in China,but its exploitation and utilization is limited at present.In order to further develop and utilize the strain resources,a wild edible fungus,No.HC-1,was taken from the willow tree on the campus of Xizang Agricultural and Animal Husbandry College in Linzhi,City,Xizang.The strain was identified as Pholiota aurivella by morphological observation,ITS sequence determination and phylogenetic analysis.The biological characteristics of the optimum growth conditions of the mycelia were studied by single factor test and orthogonal test.The results revealed that for the HC-1 strain of Pholiota aurivella,the optimal carbon source for mycelial growth was sucrose,the best nitrogen source was yeast extract,the optimal pH was 7.0,the preferred temperature was 25°C,the optimal inorganic salt was potassium dihydrogen phosphate,and the most suitable growth factor was vitamin C.Orthogonal experiments indicated that the optimal formula for mycelial growth of strain HC-1 was 25.0 g·L^(-1) sucrose,3.0 g·L^(-1) yeast extract,pH 7.0,and 2.0 g·L^(-1) magnesium sulfate.
作者
王玉珊
何建清
陈扬
潘长漭
黄秋月
张格杰
WANG Yushan;HE Jianqing;CHEN Yang;PAN Changmang;HANG Qiuyue;ZHANG Gejie(College of Plant Science,Xizang Agriculture&Animal Husbandry University,Linzhi,Xizang,860000,China)
出处
《高原农业》
2025年第1期36-46,共11页
Journal of Plateau Agriculture
基金
“新农科”高原植物生产类专业提升实践创新能力平台建设(藏财政预指2023-1号)。
关键词
金毛鳞伞
分离
ITS鉴定
生物学特性
Pholiota aurivella
Isolation
ITS identification
Biological properties
