摘要
将昆明小鼠随机分为空白对照组(NC)、模型组(PB)、广叶绣球菌多糖组(SLP)、同笼铅暴露组(Co-PB)和同笼广叶绣球菌多糖组(Co-SLP),除NC外,其余组以1 g·L^(-1)醋酸铅溶液为饮用水,SLP、CoSLP每天灌胃400mg·kg^(-1)的广叶绣球菌多糖,采用石墨炉原子吸收光谱法检测海马铅含量,采用苏木精-伊红染色法观察海马结构,测定海马丙二醛(MDA)、谷胱甘肽(GSH)含量及总超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GPx)的活性,利用实时荧光定量PCR技术检测海马中白细胞介素-4(IL-4)、白细胞介素-6(IL-6)、白细胞介素-10(IL^(-1)0)、白细胞介素-1β(IL^(-1)β)、肿瘤坏死因子-α(TNF-α)、转化生长因子-β(TGF-β)、自噬效应蛋白1(Beclin1)、微管相关蛋白轻链3-Ⅰ(LC3-Ⅰ)、微管相关蛋白轻链3-Ⅱ(LC3-Ⅱ)、溶酶体关联酶蛋白2(Lamp2)、泛素结合蛋白(P62)、自噬相关蛋白5(Atg5)基因的相对表达量。结果表明:与PB比较,SLP、Co-SLP、Co-PB的铅含量显著降低;SLP、Co-SLP的CAT和GPx活性均显著增加,SLP、Co-SLP、Co-PB的SOD活性和GSH含量均显著增加,SLP、Co-SLP、Co-PB的MDA含量显著降低;SLP的IL^(-1)β表达量显著降低,SLP、Co-SLP的IL-6表达量显著降低,SLP、Co-SLP、Co-PB的TNF-α表达量显著降低,SLP、Co-SLP的IL-4表达量显著增加,SLP、Co-SLP、Co-PB的TGF-β和IL^(-1)0表达量均显著增加;SLP、Co-SLP的LC3-II、Lamp2和Beclin1表达量均显著降低,SLP、Co-SLP、Co-PB的LC3-I和Atg5表达量均显著降低,Co-SLP的P62表达量显著增加。研究结果可为利用广叶绣球菌多糖缓解铅中毒提供参考。
Kunming mice were randomly divided into blank control(NC)group,lead exposure(PB)group,Sparassis latifolia polysaccharide(SLP)group,and lead exposure(Co-PB)group co-housed with SLP-1 treatment(Co-SLP)group.Except NC group,mice in the rest groups were given 1 g·L lead acetate solution as drinking water,and mice in SLP and Co-SLP groups were also administered 400 mg·kg^(-1) SLP by gavage.After 8 weeks,mice in different groups were measured for lead content in hippocampus by graphite furnace atomic absorption spectrometry,observed for hippocampal tissue structure by hematoxylin-eosin staining,determined for contents of malondialdehyde(MDA)and glutathione(GSH),and determined for activities of total superoxide dismutase(SOD),catalase(CAT)and glutathione peroxidase(GPx)in hippocampus.Real-time quantitative PCR was used to detect relative expression of inflammation-related genes IL-4,IL-6,IL^(-1)0,IL^(-1)β,TNF-α,TGF-β,and autophagy related genes Beclin-1,LC3-I,LC3-II,Lamp2,P62 and Atg5 in hippocampus.The results showed that the hippocampal lead contents of SLP,Co-SLP and Co-PB were significantly lower than that of PB.There were significant increases of CAT activity and GPx activity in SLP mice and Co-SLP mice compared with PB mice.The SOD activity and GSH content of SLP,Co-SLP and Co-PB groups were also increased significantly.On the other hand,the contents of MDA in SLP,Co-SLP and Co-PB groups decreased significantly compared with that in PB group.Compared with PB mice,mice treated with SLP showed significantly decreased expression of IL^(-1)β,IL-6 TNF-α,LC3II,Lamp2,Beclin1,LC3-I and Atg5,and significantly increased expression of IL-4,TGF-βand IL^(-1)0.Compared with PB mice,Co-SLP mice showed significantly decreased expression of IL-6,TNF-α,LC3 II,Lamp2,Beclin1,LC3-I and Atg5,and significantly increased expression of IL-4,TGF-β,IL^(-1)0 and P62.Compared with PB mice,Co-PB mice showed significantly decreased expression of TNF-α,LC3-I and Atg5,and significantly increased expression of TGF-βand IL^(-1)0.The results provided a reference for ameliorating lead injury by S.latifolia polysaccharide.
作者
吴蕊
冯瑞
牛旭凯
许梦燕
韩笑月
云少君
冯翠萍
曹谨玲
WU Rui;FENG Rui;NIU Xukai;XU Mengyan;HAN Xiaoyue;YUN Shaojun;FENG Cuiping;CAO Jinling(College of Food Science and Engineering,Shanxi Agricultural University,Jinzhong 030801,Shanxi,China;Shanxi Key Laboratory of Edible Fungi for Loess Plateau,Jinzhong 030801,Shanxi,China;Shanxi Provincial Aquatic Technology Extension Service Center,Taiyuan 030006,Shanxi,China)
出处
《食用菌学报》
CSCD
北大核心
2024年第6期50-58,共9页
Acta Edulis Fungi
基金
山西省基础研究计划(20210302123384)
山西省基础研究计划(202103021224126)
山西省科技创新团队(201805D131009)。
关键词
广叶绣球菌
多糖
氧化应激
海马
炎症因子
自噬基因
Sparassis latifolia
polysaccharide
oxidative stress
hippocampus
inflammatory factors
autophagy gene
