摘要
目的:探究菟丝子三阴交穴位贴敷对复发性流产大鼠妊娠结局的改善作用,及其对母胎界面Th1/Th2平衡的影响。方法:将SD雌性孕鼠随机分为正常孕鼠组、流产模型组、菟丝子穴位贴敷组、菟丝子灌胃组、淀粉穴位贴敷组,每组15只。除正常孕鼠组外,其余各组孕鼠于孕6~8 d时皮下注射溴隐亭0.3 mg/kg,1次/d,建立流产模型,并于妊娠第1天给药治疗,于妊娠第12天取腹主动脉血,检测血清催乳素(PRL)及孕酮(P)水平;处死孕鼠,取离体子宫,计算胚胎流产率并称量子宫重量,以评估孕鼠妊娠结局变化;取蜕膜组织,免疫组化法检测孕激素受体(PR)阳性表达;RT-PCR及Western blot检测蜕膜组织Th1型(TNF-α、IFN-γ)/Th2型(IL-4、IL-10)细胞因子mRNA及蛋白表达。结果:与正常孕鼠组相比,流产模型组孕鼠胚胎流产率升高,子宫重量、孕激素PRL、P及PR表达降低,蜕膜组织Th1型(TNF-α、IFN-γ)细胞因子mRNA及蛋白表达升高,而Th2型(IL-4、IL-10)细胞因子mRNA及蛋白表达降低(P<0.05)。菟丝子穴位贴敷、菟丝子灌胃治疗均可降低流产率,提高PRL、P及PR表达,纠正Th1/Th2失衡,且菟丝子穴位贴敷治疗效果优于菟丝子灌胃(P<0.05)。结论:菟丝子三阴交穴位贴敷可改善流产模型大鼠不良妊娠结局,纠正Th1/Th2失衡,降低流产率,且其作用效果优于菟丝子灌胃。
Objective:To explore the improvement effect of acupoint application of seed of Chinese dodder at Sanyinjiao acupoint on pregnancy outcome in rats with recurrent abortion,and its influence on the balance of Th1/Th2 at maternal-fetal interface.Methods:SD female pregnant rats were randomly divided into normal pregnant rats group,abortion model group,acupoint application of seed of Chinese dodder group,gavage of seed of Chinese dodder group,acupoint application of starch group,with 15 rats in each group.Except for normal pregnant rats group,pregnant rats of other groups were injected subcutaneously with 0.3 mg/kg bromocriptine on 6~8 days of gestation,for once a day,the abortion model was established,the treatment was given from the first day of pregnancy,and the blood was taken from the abdominal aorta on the 12th day of pregnancy to detect levels of serum prolactin(PRL)and progesterone(P);pregnant rats were sacrificed,the uterus was taken out,the embryo abortion rate was calculated and the uterus weight was weighed to evaluate changes of pregnancy outcome of pregnant rats;the decidua tissue was taken,and the positive expression of progesterone receptor(PR)was detected by immunohistochemical method;Th1 type(TNF-α,IFN-γ)/Th2 type(IL-4,IL-10)cytokines mRNA and protein expressions in decidua tissue were detected by RT-PCR and Western blot.Results:Compared with normal pregnant rats group,abortion model group had an increased embryo abortion rate,and decreased uterine weight,progesterone PRL,P and PR expressions,the decidual tissue Th1 type(TNF-α,IFN-γ)cytokines mRNA and protein expressions were increased,while Th2 type(IL-4,IL-10)cytokines mRNA and protein expressions were decreased(P<0.05).Acupoint application of seed of Chinese dodder and gavage of seed of Chinese dodder could reduce the abortion rate,increase expressions of PRL,P and PR,and correct the Th1/Th2 imbalance,and the therapeutic effect of acupoint application of seed of Chinese dodder is better than that of gavage of seed of Chinese dodder(P<0.05).Conclusion:Application of the seed of Chinese dodder at Sanyinjiao acupoint can improve the adverse pregnancy outcome of abortion model rats,correct Th1/Th2 imbalance,and reduce the abortion rate,and its effect is better than gavage of seed of Chinese dodder.
作者
陈雪梅
朱争艳
CHEN Xuemei;ZHU Zhengyan(Department of Gynecology,Wuhan Third Hospital,Wuhan 430074,China)
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2024年第12期2585-2590,共6页
Chinese Journal of Immunology
基金
武汉市医学科研项目(WZ21C12)。
关键词
菟丝子
三阴交穴
穴位贴敷
复发性流产
妊娠结局
TH1/TH2平衡
Seed of Chinese dodder
Sanyinjiao acupoint
Acupoint application
Recurrent abortion
Pregnancy outcome
Th1/Th2 balance
