摘要
目的分析黄芪多糖(APS)对甲状腺乳头状癌(PTC)细胞微小RNA(miRNA)26及其靶基因表达的影响。方法体外常规培养PTC TPC-1细胞,并分为TPC-1组(空白)和APS100组、APS200组、APS400组(分别用100、200、400μg/ml APS处理)。采用倒置显微镜观察细胞形态,CCK-8法检测各组TPC-1细胞活性,RT-qPCR法检测TPC-1细胞中miRNA26的相对表达量及APS的干预作用,RT-qPCR及Western blot法检测FOXO1的mRNA、蛋白质表达水平和APS的干预作用。结果不同剂量的APS能抑制TPC-1细胞的增殖,抑制时效为24 h,其中以浓度200μg/ml的抑制效果最佳;APS能下调miRNA26的表达量,上调FOXO1的mRNA及蛋白质表达水平。结论APS可能通过下调miRNA26进而负反馈调控FOXO1基因表达,以此发挥对TPC-1细胞的抑制作用。
Objective To analyze the effect of astragalus polysaccharides(APS)on the expression of microRNA(miRNA)26 and its target genes in papillary thyroid carcinoma(PTC)cells.Methods PTC TPC-1 cells were routinely cultured in vitro and divided into TPC-1 group(blank)and APS100,APS200 and APS400 groups(treated with 100,200 and 400μg/ml APS).Inverted microscope was used to observe the cell morphology,CCK-8 assay was used to detect the activity of TPC-1 cells in each group,RT-qPCR assay was used to detect the relative expression of miRNA26 in TPC-1 cells and the intervention effect of APS,RT-qPCR and Western blot were used to detect the mRNA and protein expression levels of FOXO1 and the intervention effect of APS.Results Different doses of APS inhibited the proliferation of TPC-1 cells for 24 h,with the best inhibition effect at a concentration of 200μg/ml.APS down-regulated the expression of miRNA26 and up-regulated the mRNA and protein expression levels of FOXO1.Conclusion APS may play an inhibitory role on TPC-1 cells by down-regulating miRNA26 and then negatively regulating FOXO1 gene expression.
作者
顾远晖
谢青
寇雨顺
王晓科
杨鑫
伊琳
GU Yuanhui;XIE Qing;Kou Yushun;WANG Xiaoke;YANG Xin;Yi Lin(Gansu Provincial Hospital,Gansu,Lanzhou 730000,China;Gansu University of Chinese Medicine,Gansu,Lanzhou 730000,China)
出处
《中国医药科学》
2024年第20期4-7,共4页
China Medicine And Pharmacy
基金
甘肃省自然科学基金项目(24JRRA590)
甘肃省人民医院院内科研基金临床研究类项目(23GSSYD-17)。
关键词
微小RNA
甲状腺乳头状癌
黄芪多糖
基因
miRNA
Papillary thyroid carcinoma
Astragalus polysaccharides
Gene
