摘要
为获得对茶树根腐病原菌Fusarium cugenangense具有良好拮抗效果的拮抗真菌,采用稀释涂布平板法从茶树根腐病根际土壤分离出真菌菌株,利用平板对峙法筛选出拮抗活性较高的菌株,基于形态学观察和ITS测序分析对其进行鉴定,测定其防病促生指标、抑菌谱、挥发性代谢物及难挥发性代谢物的抑菌活性,并评估拮抗菌株发酵液对茶树根腐病的抑菌能力。结果表明,筛选获得一株对茶树根腐病原菌F.cugenangense具有良好抑制效果的菌株CA5,平板对峙抑菌率为77.39%,鉴定为嗜松篮状菌Talaromyces pinophilus;菌株CA5具有产生纤维素酶、β-1,3葡聚糖酶和几丁质酶的能力,还具有解无机磷、解有机磷、解钾、固氮、产铁载体、产IAA以及产氨能力;菌株CA5对其他8种植物病原菌的抑制率均大于60%,抗菌作用具有广谱性;其挥发性代谢物和难挥发性代谢物对茶树根腐病原菌菌丝生长的抑制率分别为14.90%和44.33%,发酵液、菌悬液、无菌发酵滤液对茶树根腐病原菌菌丝生长的抑制率分别为73.42%、67.32%、25.75%;盆栽试验结果表明,菌株CA5对茶树根腐病防效达56.11%,且对茶树幼苗生长具有促进作用。综上,菌株CA5在防控茶树根腐病方面具有巨大应用潜力。
In order to obtain the antagonistic fungi with good antagonistic effect on Fusarium cugenangense,fungal strains were isolated from the rhizosphere soil of tea plants with root rot infection by dilution coating plate method,and a strain with high antagonistic activity was screened by plate confrontation method and identified based on morphological observation and ITS sequence analysis methods.The disease prevention and growth promotion property,inhibition spectrum,and inhibition activity of volatile and non-volatile metabolites were determined.The inhibition ability of fermentation broth of antagonistic strain against F.cugenangense was evaluated.The results showed that a strain CA5 with high inhibitory effect on tea root rot pathogen was obtained and identified as Talaromyces pinophilus.Its plate inhibition rate against F.cugenangense was 77.39%.The strain CA5 had the ability to produce cellulase,β-1,3 glucanase and chitinase,and also had inorganic phosphorus and organophosphate solubilities,potassium solubility and nitrogen fixation capacities,as well as the ability to produce siderophore,IAA and ammonia.Inhibition rates of strain CA5 on other 8 plant pathogens were all greater than 60%.Inhibition rates of volatile and non-volatile metabolites on mycelial growth of tea root rot pathogen were 14.90%and 44.33%,respectively.Inhibition rates of fermentation broth,suspension and sterile fermentation filtrate on mycelial growth of tea root rot pathogen were 73.42%,67.32%and 25.75%,respectively.Pot experiment showed that the control efficacy against tea root rot disease was 56.11%,and the strain had a promoting effect on the growth of tea seedlings.In conclusion,the strain CA5 has great application potential in the prevention and control of tea root rot.
作者
邓玉莲
谭琳
吉进军
李桂花
黄虹
史子涵
胡秋龙
DENG Yulian;TAN Lin;JI Jinjun;LI Guihua;HUANG Hong;SHI Zihan;HU Qiulong(Key Laboratory of Tea Science of Ministry of Education,Hunan Agricultural University,Changsha 410128,China;College of Horticulture,Hunan Agricultural University,Changsha 410128,China;College of Plant Protection,Hunan Agricultural University,Changsha 410128,China;Hunan Tea Group Co.,Ltd.,Changsha 410126,China)
出处
《中国生物防治学报》
CSCD
北大核心
2024年第4期914-926,共13页
Chinese Journal of Biological Control
基金
国家重点研发计划项目(2022YFD16000803-6)
湖南省重点研发计划项目(2022NK2051,2023NK2013)
湖南省科技创新重大项目(2021NK1020)。
关键词
茶树
根腐病
嗜松篮状菌
促生能力
生物防治
tea plant
root rot
Talaromyces pinophilus
growth promotion
biological control
