摘要
目的研究胃癌患者血清微小RNA(miR)-125b-5p、miR-144、miR-93表达水平与病理特征及预后的关系。方法回顾性选取2018年7月至2019年12月西安医学院第一附属医院收治的胃癌患者作为恶性组(80例),另选取该院同期收治的胃部良性病变患者作为良性组(93例)。统计两组血清miR-125b-5p、miR-144、miR-93表达水平,比较不同病理特征胃癌患者血清miR-125b-5p、miR-144、miR-93表达水平。胃癌患者随访3年,根据患者不同预后情况将其分为死亡组和存活组,比较死亡组和存活组血清miR-125b-5p、miR-144、miR-93表达水平。绘制受试者工作特征(ROC)曲线分析血清miR-125b-5p、miR-144、miR-93表达水平单独及三者联合检测对胃癌患者预后的预测价值。结果恶性组血清miR-125b-5p、miR-93表达水平均高于良性组,血清miR-144表达水平低于良性组,差异均有统计学意义(P<0.05)。不同TNM分期、分化程度、淋巴结转移情况、腹膜种植转移情况患者miR-125b-5p表达水平比较,差异均有统计学意义(P<0.05)。不同肿瘤最大径、TNM分期、分化程度及淋巴结转移情况、腹膜种植转移情况、脉管浸润患者血清miR-144、miR-93表达水平比较,差异均有统计学意义(P<0.05)。死亡组纳入33例患者,存活组纳入47例患者。死亡组血清miR-125b-5p、miR-93表达水平均高于存活组,miR-144表达水平低于存活组,差异均有统计学意义(P<0.05)。ROC曲线分析结果显示,血清miR-125b-5p、miR-144、miR-93单独检测预测胃癌患者预后的曲线下面积分别为0.737、0.757、0.738,均低于三者联合检测的0.887(Z=2.123、2.362、2.308,P<0.05)。结论胃癌患者、胃部良性病变患者及不同病理特征、不同预后胃癌患者血清miR-125b-5p、miR-144、miR-93表达水平存在明显差异,血清miR-125b-5p、miR-144、miR-93联合对胃癌患者预后具有较好的预测价值,有望成为预测胃癌患者预后的标志物。
Objective To investigate the relationship between the expression levels of serum microRNA(miR)-125b-5p,miR-144 and miR-93 and the pathological characteristics and prognosis of patients with gastric cancer.Methods Patients with gastric cancer admitted to the First Affiliated Hospital of Xi′an Medical University from July 2018 to December 2019 were retrospectively selected as the malignant group(80 cases),and patients with benign stomach lesions admitted to the hospital during the same period were also selected as the benign group(93 cases).The expression levels of serum miR-125b-5p,miR-144 and miR-93 in patients with gastric cancer with different pathological characteristics were analyzed and compared.Patients with gastric cancer were followed up for 3 years and divided into death group and survival group according to different prognosis,and the expression levels of serum miR-125b-5p,miR-144 and miR-93 in death group and survival group were compared.Receiver operating characteristic(ROC)curve was drawn to analyze the predictive value of serum miR-125b-5p,miR-144,and miR-93 expression levels alone and combined in patients with gastric cancer.Results The expression levels of serum miR-125b-5p and miR-93 in the malignant group were higher than those in the benign group,and the expression level of serum miR-144 was lower than that in the benign group,with statistical significance(P<0.05).Serum miR-125b-5p expression levels in patients with different TNM stage,differentiation degree,lymph node metastasis and peritoneal implantation metastasis had statistical significance(P<0.05).There were statistically significant differences in the expression levels of miR-144 and miR-93 in serum among patients with different tumor size,TNM stage,differentiation degree,lymph node metastasis,peritoneal implantation metastasis,and vascular infiltration(P<0.05).There were 33 patients in the death group and 47 patients in the survival group.The expression levels of miR-125b-5p and miR-93 in the death group were higher than those in the survival group,and the expression levels of miR-144 were lower than those in the survival group,with statistical significance(P<0.05).ROC curve analysis results showed that the area under the curve of serum miR-125b-5p,miR-144 and miR-93 to predict the prognosis of gastric cancer patients were 0.737,0.757 and 0.738 respectively,all of them were lower than 0.887(Z=2.123,2.362,2.308,P<0.05).Conclusion There are significant differences in the expression levels of serum miR-125b-5p,miR-144 and miR-93 in patients with gastric cancer,patients with benign stomach lesions and patients with different pathological characteristics and different prognosis.The combination of serum miR-125b-5p,miR-144 and miR-93 has a good predictive value for the prognosis of patients with gastric cancer.It is expected to be a prognostic marker for patients with gastric cancer.
作者
燕普
张永康
蔡瑜
常小伟
YAN Pu;ZHANG Yongkang;CAI Yu;CHANG Xiaowei(Department of General Surgery,the First Affiliated Hospital of Xi′an Medical University,Xi′an,Shaanxi 710077,China;Department of Gastrointestinal Surgery,Xiangyang Central Hospital,Xiangyang,Hubei 441021,China)
出处
《检验医学与临床》
CAS
2024年第20期2998-3002,共5页
Laboratory Medicine and Clinic
基金
陕西省教育厅专项科研计划项目(20JK0887)。
