摘要
制备NiBM并将其应用无酶传感器的构筑,建立比色法快速检测马拉硫磷。合成的NiBM能将无色的3,3′,5,5′-四甲基联苯胺氧化为蓝色的TMB氧化物。当马拉硫磷存在时,由于NiBM中镍原子与马拉硫磷的硫代磷脂键之间具有高亲和力,NiBM与马拉硫磷相结合,抑制NiBM对TMB的氧化反应。该无酶NiBM传感器在最佳检测条件下表现出优异的稳定性和较高的检测性能,检测马拉硫磷的线性范围为0~100 ng·mL^(-1),检测限低至7.8 ng·mL^(-1)。所提出的比色传感器为有机磷农药的测定提供了一种有前景的快速检测技术,该技术使用的NiBM制备过程简单且原料廉价易得,有望应用于复杂基质中马拉硫磷的快速检测和通量筛查。
The enzyme-free colorimetric sensor was designed for the rapid detection of malathion based on NiBM.In the presence of NiBM,3,3′,5,5′-tetramethylbenzidine can be oxidized to blue oxidized TMB.When malathion was present,the prepared NiBM can bind with malathion because of the high affinity between the Ni element and the sulfur moiety in malathion,resulting in the oxidation of TMB inhibited by malathion.The enzyme-free malathion sensor exhibits excellent stability and good performance under optimal experimental conditions,with a linear range of malathion from 0 to 100 ng·mL^(-1)and a detection limit as low as 7.8 ng·mL^(-1).The preparation of NiBM was simple and economic,and the proposed sensor provides a promising technique for rapid and sensitive determination of organophosphorus pesticides in complex matrix.
作者
严喜鸾
穆鸿仪
肖义陂
郭亮
李娜
艾凡荣
YAN Xiluan;MU Hongyi;XIAO Yipi;GUO Liang;LI Na;AI Fanrong(School of Chemistry and Chemical Engineering,Nanchang University,Nanchang 330031,China;School of Pharmacy,Nanchang University,Nanchang 330031,China;School of Advanced Manufacturing,Nanchang University,Nanchang 330031,China;Sino-German Joint Research Institute,Nanchang University,Nanchang 330031,China;Nanchang Hongdu Hospital of TCM,Nanchang 330006,China)
出处
《南昌大学学报（理科版）》
CAS
2024年第4期338-344,共7页
Journal of Nanchang University(Natural Science)
基金
国家自然科学基金资助项目(82360857)
江西省自然科学基金资助项目(20212BAB206093)
江西省自然科学基金资助项目(20232BAB205087)
江西省自然科学基金资助项目(20224BAB216116)
江西省中医药科技计划资助项目(2021A321)。
关键词
镍基材料
马拉硫磷
无酶
比色法
nickel-based materials
malathion
enzyme-free
colorimetric
