摘要
采用高效液相色谱法测定天然烟用香料乌梅粉体中柠檬酸含量,优化前处理条件及色谱条件,用0.22μm的水相针式过滤器过滤萃取液,使用SunFire C_(18)色谱柱(250 mm×4.6 mm,5μm)进行分离,以甲醇-0.01 mol/L磷酸二氢钾(pH 2.7)流动相等度洗脱,用二极管阵列检测器检测,色谱峰面积外标法进行定量。方法检出限4.02 mg/g,定量限13.40 mg/g,在标准曲线质量浓度范围0.2~4 mg/mL之间,相关系数大于0.999。日间及日内相对标准偏差均小于1%(n=6),在100,200和500 mg/g这3个水平的加标下,加标回收率为95.00%~103.50%。该方法前处理简单、精密度高、定量结果准确,可为乌梅粉体中柠檬酸含量的测定提供依据。
The citric acid content in natural tobacco flavoring dark plum powder is determined by high-performance liquid chromatography(HPLC).The pretreatment conditions and chromatographic conditions are optimized.The extraction liquid is filtered by aqueous phase needle filter of 0.22μm and separated by SunFire C_(18) column(250 mm×4.6 mm,5μm).The liquid is eluted with methanol and 0.01 mol/L potassium dihydrogen phosphate(pH 2.7),detected by diode array detector,and quantified by peak area external standard method.The detection limit of the method is 4.02 mg/g,the quantitative limit is 13.40 mg/g,and the correlation coefficient is greater than 0.999 within the standard curve concentration range of 0.2-4 mg/mL.The relative standard deviation during and within the day is less than 1%(n=6).At the spiking levels of 100,200 and 500 mg/g,the spiking recovery rate is 95.00%to 103.50%.This method has simple pre-treatment,high precision,and accurate quantitative results,which can provide a basis for the determination of citric acid in dark plum powder.
作者
李美红
张文军
朱婷
秦琦
陈华
李淳皓
LI Meihong;ZHANG Wenjun;ZHU Ting;QIN Qi;CHEN Hua;LI Chunhao(China Tobacco Schweitzer(Yunnan)Reconstituted Tobacco Co.,Ltd.,Yuxi 653100)
出处
《食品工业》
CAS
2024年第9期307-310,共4页
The Food Industry
基金
云南中烟工业有限责任公司科技计划项目“超微粉体在再造烟叶微观结构中的构效关系研究及应用”(2023YL02)
中烟施伟策(云南)再造烟叶有限公司科技项目“基于原料品类的加热烟草芯材专用香原料关键技术开发”(KY24ZL03)。
关键词
再造烟叶
乌梅粉体
柠檬酸含量
高效液相色谱法
reconstituted tobacco
dark plum powder
citric acid content
high-performance liquid chromatography
