摘要
目的建立可同时测定党参中党参苷I、党参炔苷宁、党参炔苷和白术内酯III等4种成分含量的高效液相色谱(HPLC)方法。方法采用HPLC,V-0203052-5250 Svea(4.6 mm×250 mm,5µm)色谱柱,流速为0.8 ml/min,梯度洗脱,流动相为0.1%磷酸水-乙腈,检测波长为220 nm(党参苷I、白术内酯III)及210 nm(党参炔苷宁、党参炔苷),柱温25℃。结果党参苷I、党参炔苷宁、党参炔苷、白术内酯III分别在3.13~200.00,12.50~400.00,6.25~200.00,0.39~12.50µg/ml范围内线性关系良好(r^(2)≥0.9999,n=6);平均回收率为82.20%~98.94%。结论该方法可同时测定党参中4种成分的含量,重复性高,准确性好,可用于党参的质量控制。
Objective To establish an HPLC method for simultaneous determination of tangshenosideⅠ,lobetyolin and atractylenolideⅢin Codonopsis Radix.Methods HPLC was performed on V-0203052-5250 Svea(4.6 mm250 mm,5µm)column with 0.1%phosphoric acid solution-acetonitrile as mobile phases for gradient elution.The detection wavelengths were set at 220 nm(for detection of tangshenosideⅠand atractylenolideⅢ)and 210 nm(for dection of lobetyolinin and lobetyolin),respectively.The flow rate was 0.8 ml/min.The column temperature was 25℃.Results The linear relationships of tangshenosideⅠ,lobetyolinin,lobetyolin and atractylenolideⅢwere good(r^(2)≥0.9999,n=6)within their ranges of 3.13-200.00,12.50-400.00,6.25-200.00,0.39-12.50µg/ml,respectively.The average recoveries were 82.20%-98.94%.Conclusion The method is of high repeatability and good accuracy,and can be used for quality control of Codonopsis Radix and its products.
作者
王梦花
孔文茹
王智璠
张舒凯
王姝麒
WANG Meng-hua;KONG Wen-ru;WANG Zhi-fan;ZHANG Shu-kai;WANG Shu-qi(School of Pharmaceutical Sciences,Shandong University,Jinan 250012,China)
出处
《食品与药品》
CAS
2024年第5期454-457,共4页
Food and Drug
